Methods of purifying allergen extract

Abstract

The invention relates to processes for producing semi-purified and purified allergen extracts and pharmaceutical compositions and vaccines for use in the diagnosis and treatment of allergy. In one aspect of the invention, a process for producing a depigmented allergen extract is provided, the process comprising: a) basifying a native allergen extract; and b) removing molecules having a molecular size of less than 3.5 kDa; and c) adjusting the pH to neutrality; thereby to produce a depigmented allergen extract.

Description

technical field

[0001] The present invention relates to methods for the production of semi-purified and purified allergen extracts as well as pharmaceutical compositions and vaccines for the diagnosis and treatment of allergies. Background technique

[0002] Allergies are acquired hypersensitivity disorders of the immune system and are triggered by exposure to innocuous environmental substances called allergens. Type I hypersensitivity is the hallmark of allergic reactions and results in the production of excess IgE antibodies, which activate basophils and mast cells, causing an inflammatory response. Effects may be systemic, such as vasodilation, mucus secretion, nerve stimulation, or smooth muscle contraction, leading to an allergic reaction, or effects may be limited to specific areas of the body, such as the respiratory system.

[0003] Phleum pratense

[0004] Grass allergy is one of the most common and prevalent forms of allergy affecting sensitive people during cert...

Images