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Virus removal and inactivation treatment process for preparation of dust mite allergen

A treatment process and allergen technology, applied in the field of virus removal and inactivation treatment process, can solve the problems that have not been paid attention to

Inactive Publication Date: 2006-04-26
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the virus inactivation process in the preparation of allergen infusion has not been paid attention to

Method used

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  • Virus removal and inactivation treatment process for preparation of dust mite allergen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of dust mite allergen infusion:

[0030] Collect house dust mites, weigh the degreasing material, add sodium bicarbonate-saline extract in a ratio of 1:5 (1 g of house dust is added to 5 ml of extract), and extract for 72 hours. Shake on a shaker for 2 hours a day, the rest of the time in the refrigerator for extraction.

[0031] After the extraction is completed, first filter with multi-layer gauze, discard the precipitate, and then use a Buchner funnel and multi-layer filter paper to filter under reduced pressure to clarify the liquid. The amount of filtrate was recorded and put into a dialysis capsule for 24 hours. Sodium bicarbonate-saline extract was used as the dialysis fluid, changing every 6 hours. After the dialysis is completed, weigh the filtrate again. If the filtrate exceeds the original amount or is less than the original amount due to salting out, concentrate or dilute it to keep the original amount. Correct the pH of the solution to keep i...

Embodiment 2

[0034] Dry heat inactivation of viruses:

[0035] The equipment used in the extraction process was dry-baked at 300°C for 24 hours, the medium and extraction buffer were heated at 80°C for 72 hours, and the dry-heat box used for virus inactivation was verified at least every six months. During verification, multiple temperature measurement points should be set in the drying box (including the highest and lowest temperature points in the product and in the box). All operations are strictly aseptic.

Embodiment 3

[0037] Filtration to remove viruses: Filter integrity should be tested before and after filtration. A new filter is required for each filtration. Since the molecular weight of allergen proteins and polypeptides in the extract is less than 100kDa, ViresolveTM composite membrane produced by Millipore Company or PLANOVA produced by Asahi Company is used to filter viruses with a molecular weight greater than 100kDa.

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Abstract

The present invention relates to the virus elimination and deactivation process in preparing dermatophagoide allergen immersion, and viruses are eliminated and deactivated by means of several combined methods. Specifically, the combined process includes: 1.dry heating process including dry roasting of the instrument at 300 deg.c, heating culture medium and extracting buffer liquid at 80 deg.c for 72 hr, verifying the dry heating case for deactivating virus at least every half year and performing bacteria-free operation; 2. filtering process to filter allergen extracting liquid with filtering membrane to eliminate viruses of molecular weight greater than 100 KDa; 3. ultrasonic process to deactivate viruses via single cyclic path exposed to microwave energy; 4. chromatographic process to eliminate viruses of molecular weight greater than 100 KDa with Sephadex S-100 molecular sieve; and 5. verifying all the virus eliminating and deactivating processes.

Description

technical field [0001] The invention relates to an allergen extraction and preparation process, in particular to a virus removal and inactivation treatment process in the preparation of a dust mite allergen infusion. Background technique [0002] In the past 50 years, the prevalence of allergy has risen sharply in the world, and it is estimated that more than 40% of the global population is atopic. Asthma has a prevalence rate of 10% to 15% in children, and there are 100 to 150 million children worldwide. Asthma is the main type of hospitalization for children with chronic diseases in Western countries, and many countries pay huge health expenses for this. The 1997 European Allergy White Paper pointed out the prevalence of allergies in the 21st century and the prevention of the rising prevalence of allergy / asthma. In 2000, the World Allergy Organization (WAO) was established to be responsible for the exchange of allergy. and education, discuss prevention strategies. [000...

Claims

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Application Information

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IPC IPC(8): C12N7/04
Inventor 刘志刚吉坤美喻海琼
Owner SHENZHEN UNIV
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