Lactobacillus gasseri LS03 and application thereof

A technology of Lactobacillus gasseri and bacterial suspension, applied in the field of microorganisms, can solve the problems of dependence on imports of probiotic strains, influence on the promotion of probiotics and their products, lack of scientific research evidence for probiotic functions, etc., and achieve good reproductive tract infection. , the prevention or alleviation of reproductive tract infection, the effect of alleviation or prevention of female reproductive tract infection

Pending Publication Date: 2021-12-31
广东一元兰欣生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The probiotic strains used by domestic production enterprises have long relied on imports, and foreign strains may not be suitable for the physiological conditions of my country's

Method used

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  • Lactobacillus gasseri LS03 and application thereof
  • Lactobacillus gasseri LS03 and application thereof
  • Lactobacillus gasseri LS03 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1 Lactobacillus gasseri LS03 isolation, identification and preservation

[0024] (1) Separation: The reproductive tract secretions of healthy women of childbearing age were serially diluted, inoculated on anaerobic blood agar medium and MRS solid medium respectively, cultured anaerobically at 37°C for 48 hours, and picked single colonies on the plate to mark Line isolation yielded pure colonies. The pure colony on the plate was inoculated into MRS liquid medium, cultured anaerobically at 37°C for 12-16h, added 20% glycerol, and stored in a -80°C refrigerator.

[0025] (2) Morphological identification of bacterial strains: Gram-stained strains were observed under a microscope, Gram-positive bacteria were purple, and Gram-negative bacteria were red.

[0026] (3) Molecular biological identification of the strain: Genomic DNA was extracted from the obtained strain, and the full-length 16S rDNA fragment was amplified by PCR using 16S rDNA universal primers 27F and 1...

Example Embodiment

[0033] Example 2 Lactobacillus gasseri LS03 Acid Production Ability Determination

[0034] Lactobacillus gasseri LS03 was inoculated in MRS liquid medium, cultured in an anaerobic workstation at 37°C for 14 hours, and the strain concentration was adjusted to 1×10 6 CFU / mL, inoculated in fresh MRS liquid medium at 5% volume fraction, placed in a full-temperature shaker at 37°C and 150r / min for 0, 5, 10, 15, 20, 24, 28, 32, 36 After 48 hours, the concentration of lactic acid in the fermentation supernatant of the strain was detected by Hitachi automatic biochemical analyzer. At the same time, a pH meter (Sartorius Scientific Instruments (Beijing) Co., Ltd.) was used to measure the pH value of the fermentation broth.

[0035] see results image 3 and Figure 4 . Depend on image 3 It can be seen that Lactobacillus gasseri LS03 started to ferment and produce lactic acid after being inoculated in the medium, and almost reached the saturated concentration after 24 hours, and th...

Example Embodiment

[0036] Preparation of embodiment 3 Lactobacillus gasseri LS03 fermentation supernatant (extracellular secretion), bacterial suspension (thalline)

[0037] Lactobacillus gasseri LS03 was activated and cultured and inoculated in MRS liquid medium. After culturing at 37°C for 15 hours, the concentration of fermentation bacteria was adjusted to 1×10 7 CFU / mL, centrifuged at 4°C, 6000r / min for 10min to obtain the culture supernatant and cell pellet, the supernatant was filtered through a 0.22μm filter membrane to obtain the fermentation supernatant (extracellular secretion); the cell pellet was filtered through PBS twice After the first wash, the cells were resuspended with PBS, and the cell concentration was adjusted to 1×10 7 CFU / mL to obtain bacterial suspension (bacteria).

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Abstract

The invention discloses lactobacillus gasseri LS03 and application thereof, and belongs to the technical field of microorganisms. The invention discloses a strain of lactobacillus gasseri LS03, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 22009. Lactobacillus gasseri LS03 can produce lactic acid to reduce the pH value; and meanwhile, the growth of pathogenic bacteria can be remarkably inhibited in vitro, the adhesion of escherichia coli, staphylococcus aureus and candida albicans to human vaginal epithelial cells VK2/E6E7 can be remarkably inhibited, and the lactobacillus gasseri LS03 can be used for preparing medicines for treating and/or preventing female genital tract diseases. The lactobacillus gasseri LS03 disclosed by the invention has a huge potential application prospect in the aspect of relieving and/or preventing female genital tract diseases.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and more specifically relates to a strain of Lactobacillus gasseri LS03 and its application. Background technique [0002] Worldwide, female reproductive tract disease is a major global social and public health problem, and female reproductive tract disease is also one of the common diseases affecting female reproductive health and gynecology. In recent years, the incidence of female reproductive tract diseases in the world has been increasing year by year, seriously affecting people's quality of life. The female reproductive tract is an open cavity, and a large number of microorganisms are distributed in the female reproductive tract, especially the lower reproductive tract composed of the vagina and cervix, forming a unique micro-ecological environment. Different from reproductive tract malignant tumors that threaten women's lives, patients with infectious diseases usually complain of "...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P15/02A61P31/04A61P31/10C12R1/225
CPCA61K35/747A61P15/02A61P31/04A61P31/10Y02A50/30
Inventor 张召郑康帝陈涛
Owner 广东一元兰欣生物科技有限公司
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