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Element gamma-glutamyl transpeptidase for high expression of extracellular protein in bacillus licheniformis, method and application

A technology of Bacillus licheniformis and glutamyl transpeptidase, which is applied in the field of component γ-glutamyl transpeptidase, can solve problems such as undiscovered patent publications, and achieve purification, save time and labor, and improve expression and the cumulative effect

Pending Publication Date: 2022-01-14
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0005] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Element gamma-glutamyl transpeptidase for high expression of extracellular protein in bacillus licheniformis, method and application
  • Element gamma-glutamyl transpeptidase for high expression of extracellular protein in bacillus licheniformis, method and application
  • Element gamma-glutamyl transpeptidase for high expression of extracellular protein in bacillus licheniformis, method and application

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Embodiment Construction

[0041] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0042] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0043] An element gamma-glutamyl transpeptidase highly expressed in extracellular proteins in bacillus licheniformis, the gene sequence of the gamma-glutamyl transpeptidase is: SEQ ID NO.3.

[0044] Preferably, the γ-glutamyl transpeptidase is derived from Bacillus licheniformis 2709.

[0045] The application of the above-mentioned element γ-glutamyl transpeptidase for high expression of extracellular protein in Bacillus licheniformis in high expression of extracellular protein.

[0046] The application of the γ-glut...

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Abstract

The invention discloses an element gamma-glutamyl transpeptidase for high expression of extracellular protein in bacillus licheniformis. The gene sequence of the gamma-glutamyl transpeptidase is SEQ ID NO.3. According to the invention, a strain B.licheniformis 2709 is taken as a starting strain, green fluorescent protein eGFP is introduced as a reporter gene, mutant strain screening is carried out on bacillus licheniformis, and a strain with high fluorescence intensity is screened out for fermentation culture. The high-yield secretory protein in the mutant strain is analyzed and researched, and an element capable of performing high expression of extracellular protein is screened out. And a reference basis is provided for realizing efficient and controllable expression of other subsequent genes in the system.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to an element gamma-glutamyl transpeptidase for high expression of extracellular proteins in bacillus licheniformis, a method and an application. Background technique [0002] γ-glutamyltranspeptidase (γ-glutamyltranspeptidase, GGT) is a specific enzyme widely distributed in microorganisms, which can specifically catalyze the transfer of γ-glutamyl group, and is used in the synthesis of γ-glutamyl compounds. It has important application value. And γ-glutamyl transpeptidase has dual catalytic properties. It can not only specifically catalyze the breaking of the γ-glutamyl bond in the γ-glutamyl compound, release glutamic acid, which is manifested as hydrolysis; at the same time, it can transfer the γ-glutamyl group to the receptor to generate new γ-Glutamyl compounds exhibit transpeptidation. In view of its dual catalytic properties, γ-glutamyl transpeptidase has a very broad ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12N15/54C12N15/113C12N15/67C12N15/65C12N15/75C12R1/10
CPCC12N9/104C12N15/67C12N15/65C12N15/75C12Y203/02002
Inventor 张会图房红磊路福平
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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