Bioactivity of dehydrocostus lactone on spodoptera frugiperda and application of dehydrocostus lactone in agricultural control
A technology for dehydroxylactone, Spodoptera frugiperda, applied in application, animal husbandry, biocide and other directions, can solve problems such as resistance and pesticide pollution
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[0017] Below by embodiment the present invention will be further described. It should be understood that the methods described in the embodiments of the present invention are only used to illustrate the present invention, rather than limit the present invention, and any simple improvement of the present invention under the premise of the concept of the present invention belongs to the protection scope of the present invention.
[0018] 1. Experimental reagents and instruments
[0019] Insect cells for testing: Spodoptera frugiperda ovary cell line Sf9, using Grace insect cell culture medium, adding 8% fetal bovine serum, 1% double antibody (100unit / mL penicillin and 100μg / mL streptomycin), Cultured at 28°C in a constant temperature incubator.
[0020] Insects tested: Spodoptera frugiperda was collected from the summer corn field of Banqiao Village, Dafan Town, Tongshan County, Hubei Province on July 20, 2019. Artificial feed was used for subculture breeding indoors, adults w...
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