Polypeptide for killing tumor cells and application thereof

A technology of tumor cells and amino acids, applied in the direction of anti-tumor drugs, medical preparations containing active ingredients, peptides, etc., to achieve the effect of solving large side effects

Active Publication Date: 2022-03-04
哈尔滨吉象隆生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Among the existing main therapeutic drugs, there is a lack of a drug that has a clear effect and can be naturally degraded in the human body at an appropriate time. After killing the tumor tissue strongly, it has little side effects and almost no impact on the human body.

Method used

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  • Polypeptide for killing tumor cells and application thereof
  • Polypeptide for killing tumor cells and application thereof
  • Polypeptide for killing tumor cells and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1. A kind of synthetic method of the polypeptide that kills tumor cell

[0067] One, a kind of synthetic method of antibacterial polypeptide

[0068] 1. Based on FMOC synthesis method, adopt solid phase synthesis method, use amide resin as solid phase carrier (crosslinking degree 11%, 0.97mmol / g), special amino acid protected by FMOC, HOBT / DIC coupling agent, 10 % piperidine / DMF (volume fraction) organic base, according to the amino acid feeding of 3 times the synthesis scale, connect KLLKK LLKKL LKKLL KK sequentially according to the sequence.

[0069] 2. Use phenol: TA: EDT: water: TIS: trifluoroacetic acid = 5:5:5:4:1:80 (volume ratio) as the shearing agent, stir and avoid light to react. Suction filtration after the reaction, the filtrate precipitated, suction filtration again, the filter cake was the target peptide.

[0070] 3. The crude peptide synthesized was separated and purified by preparative reversed-phase high-performance liquid chromatography ...

Embodiment 2

[0113] Embodiment 2. The application of preparing PDC

[0114] The polypeptide of the present invention is mainly used in two aspects of interventional therapy and targeted drug delivery.

[0115] Interventional therapy: The drug delivery catheter is inserted from the tumor tissue close to the artery, and the catheter port is close to the tumor tissue. Multiple administrations or continuous administration can kill tumor tissue.

[0116] Targeted drug delivery: In the research of PDC drugs, the polypeptide structure of the present invention replaces chemotherapy drugs as payloads to kill tumor cells, and the polypeptides are naturally degraded after the treatment is completed. Due to the concentration dependence, the concentration of the drug that is not enriched in the tumor tissue is very low, does not produce drug effect, has almost no effect on the human body, and has almost no side effects.

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Abstract

The invention discloses a polypeptide for killing tumor cells and application thereof, and belongs to the technical field of molecular biology. The invention aims to provide a polypeptide with strong concentration-dependent targeting property and strong stability. The sequence structure of the polypeptide is a + n (b) + a, a is any one amino acid or multiple amino acids in KKLL, b is KKLL, and n is an integer of 1-10. The larger the molecular weight of the polypeptide drug for killing tumor cells is, the stronger the drug effect is, and the smaller the molecular weight of the drug is, the smaller the drug effect is. In tumor treatment, the compound can be applied to drugs for killing tumor cells in a special administration mode or treatment schemes of special administration equipment.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a polypeptide for killing tumor cells and its application. Background technique [0002] The World Health Organization's International Agency for Research on Cancer (IARC) released the latest global cancer burden data for 2020. Estimated the latest incidence, mortality, and cancer development trends of 36 cancer types in 185 countries around the world. According to the latest estimates, there will be 19.29 million new cancer cases worldwide in 2020, including 10.06 million males and 9.23 million females; 9.96 million cancer deaths worldwide in 2020, including 5.53 million males and 4.43 million females . There are 2.26 million new cases of breast cancer in the world, exceeding the 2.2 million cases of lung cancer. Breast cancer has replaced lung cancer and become the largest cancer in the world. In 2020, there will be 4.57 million new cancer cases in China...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K7/08A61K38/10A61K38/08A61P35/00
CPCC07K7/06C07K7/08A61P35/00A61K38/00
Inventor 冷宁苏宏健李晓鹏关伟萍田辉杨文龙王丽莉吴霜
Owner 哈尔滨吉象隆生物技术有限公司
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