Oxidoreductase for improving electrochemical activity and biosensor containing oxidoreductase

A biosensor and reductase technology, applied in the field of electrochemistry, can solve problems such as difficult and precise control of preparation, uncertainty of performance of glucose monitoring system, etc., and achieve the effect of improving catalytic oxidation efficiency

Pending Publication Date: 2022-03-08
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the redox mediator is a small molecule or a polymer material, it is difficult to precisely control its preparation. At the same time, there is also the possibility that the redox mediator may seep out of the implantable glucose biosensor. The performance of the monitoring system introduces considerable uncertainty

Method used

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  • Oxidoreductase for improving electrochemical activity and biosensor containing oxidoreductase
  • Oxidoreductase for improving electrochemical activity and biosensor containing oxidoreductase
  • Oxidoreductase for improving electrochemical activity and biosensor containing oxidoreductase

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Experimental program
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Effect test

Embodiment 1

[0061] First, 2 mg / mL glucose oxidase was incubated in PBS buffer solution containing 3 mol / L urea (preferred value: 3) at 4° C. for 12 hours to fully develop the glucose oxidase. Then mix 2mg / mL of ruthenium or osmium complex with free amino group with 2mg / mL of glucose oxidase, then add 2mmol / L of carbodiimide and 0.5mmol / L of N-hydroxysuccinyl Imine, after mixing well, reacted at 4°C for 12h. Then, the modified perglucose oxidase was separated and purified by ultrafiltration bag dialysis (cut molecular weight: 10000).

Embodiment 2

[0063] First, incubate 1 mg / mL glucose oxidase in PBS buffer solution containing 8 mol / L urea at 4°C for 24 hours to fully develop the glucose oxidase. Then mix 1 mg / mL of ruthenium or osmium complex with free amino group with 10 mg / mL of glucose oxidase, and then add 1 mmol / L of carbodiimide and 1 mmol / L of N-hydroxysuccinyl Imine, after mixing well, reacted at 4°C for 24h. Then, the modified perglucose oxidase was separated and purified by ultrafiltration bag dialysis (cutting molecular weight: 30000).

Embodiment 3

[0065] First, incubate 10 mg / mL glucose oxidase in PBS buffer solution containing 1 mol / L urea at 4°C for 18 hours to fully develop the glucose oxidase. Then mix 10 mg / mL of ruthenium or osmium complex with free amino groups with 1 mg / mL of glucose oxidase, and then add 10 mmol / L carbodiimide and 0.1 mmol / L N-hydroxysuccinate in sequence Imide, after mixing well, reacted at 4°C for 18h. Then, the modified perglucose oxidase was separated and purified by ultrafiltration bag dialysis (cut molecular weight: 1000).

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Abstract

The invention relates to the field of electrochemistry, in particular to oxidoreductase for improving electrochemical activity and a biosensor containing the oxidoreductase. The applicant finds that the chemically cross-linked glucose oxidase still keeps the direct electrochemical activity of the glucose oxidase, for example, a glutaraldehyde chemically cross-linked glucose biosensor containing the modified glucose oxidase shows good electrochemical performance on an electrode, and is a typical surface electrochemical phenomenon. The experimental results prove that chemical crosslinking has no obvious influence on the glucose oxidase after electrochemical activation, which paves a way for the application of the glucose oxidase after electrochemical activation in an implantable continuous glucose monitoring system.

Description

technical field [0001] The invention relates to the field of electrochemistry, in particular to an oxidoreductase for improving electrochemical activity and a biosensor containing the oxidoreductase. Background technique [0002] Since Clark and Lyon successfully developed the first biosensor in 1962, after more than 50 years of development, biosensors have been widely used in environmental detection, food industry, clinical medicine and other fields. For example, various glucose sensors developed based on biosensing technology have benefited millions of diabetic patients. Among them, the implantable continuous glucose monitoring system developed rapidly in recent years is favored by more and more diabetic patients, especially type I diabetic patients, due to its convenient use and real-time monitoring. As the core component of the implantable continuous glucose monitoring system, the performance of the glucose biosensor directly determines the performance and service life ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48C12Q1/00
CPCG01N27/3277G01N27/48C12Q1/004C12Q1/006G01N2333/904
Inventor 高志强
Owner SINOCARE
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