Mortierella alpina YW25, culture method thereof, fungicide, application of fungicide and method for promoting growth of araliaceae plants
A technology of Mortierella alpine and a culturing method is applied in the field of microorganisms to achieve the effect of increasing the content of available nitrogen and promoting plant growth
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[0033] In the present invention, the Mortierella alpina YW25 is preferably obtained through activation; the medium used in the activation is preferably a PDA medium; the preparation method of the PDA medium preferably includes the following steps: take the peeled Potatoes 20g were boiled for 20 minutes to get the juice, and the juice was mixed with 2g glucose, 1.5g agar and water to make 100mL; the pH of the PDA medium was preferably 6.8~7.0; the temperature of the activation was preferably 30°C; The time is preferably 5 days; the amount of inoculum during the activation is preferably 1 to 3 bacterial blocks with a diameter of 5 mm per 100 mL of medium, more preferably 1 to 2, and most preferably 1.
[0034] The invention provides a fungicide for promoting the growth of Araliaceae plants. The active ingredient of the fungicide includes the above-mentioned Mortierella alpina YW25. In the present invention, the active ingredient in the bacterial agent is preferably a suspension ...
Embodiment 1
[0042] The separation and purification of embodiment 1 bacterial strain I
[0043] The composition of the PDA plate is: 200 g of peeled potatoes, 20 g of glucose, 20 g of agar and 1000 mL of water.
[0044] Take 10 g of ginseng rhizosphere soil, put it into a triangular flask filled with 90 mL of sterile water, shake it for 20 minutes, and obtain the soil dilution;
[0045] Spread 200 μL of the soil dilution on a PDA plate and place it in a 30° C. incubator for 5 days.
[0046] Pick and inoculate a single colony grown on a new PDA plate to continue culturing for 5 days at a temperature of 30°C, then repeatedly inoculate a single colony on a new PDA plate for 5 days. The culture temperature is 30°C until there are no bacteria on the plate;
[0047] After culturing on the PDA plate for 7 days, strain I was obtained, and the colony morphology of the strain was as follows: figure 1 As shown, the colony is white, the surface is flat, velvet-like, and grows in lobes.
Embodiment 2
[0048] Identification of embodiment 2 bacterial strain I
[0049] The DNA of strain I was extracted using the D2300 Fungal Genomic DNA Extraction Kit of Solarbio Company, and entrusted Harbin Qingke Biological Co., Ltd. to perform sequencing and identification, and determine the ITS sequence, that is, the sequence of the transcribed spacer. The measured sequence is shown in SEQ ID No.1; The similarity rates of closely related species are shown in Table 1, wherein the similarity rates in Table 1 are calculated using the method of Highly similarsequences (megablast) in NCBI. It can be seen that the similarity rate between this strain and Mortierella alpina JZ-157 and Mortierella alpina OVR3 reaches 99.22%, and the similarity rate with Mortierella alpina OVR3 is 99.22%. The similarity rate of alpina LZ15-01 is 98.92%. At the same time, the phylogenetic tree was constructed on MEGA7 according to the maximum likelihood method. For details, see figure 2 , and found that the strain ...
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