Rapid automatic focusing method of microscope

An automatic focus and microscope technology, applied in the microscope field, can solve problems such as inability to focus, and achieve the effects of saving focusing time, ensuring focusing speed, and ensuring focusing accuracy

Active Publication Date: 2022-03-15
重庆切克威科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In view of this, the purpose of the present invention is to provide a rapid auto-focusing method for a microscope to solve the technical probl

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  • Rapid automatic focusing method of microscope
  • Rapid automatic focusing method of microscope
  • Rapid automatic focusing method of microscope

Examples

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Example Embodiment

[0022] The present invention will be further described below in conjunction with the examples.

[0023] The fast automatic focusing method of the microscope in this embodiment includes firstly controlling the coarse focal-focus screw to drive the stage to move up and down to adjust the focal length, and then to control the fine focal-focus screw to drive the stage to move up and down to finely adjust the focal length.

[0024] Controlling the lifting and lowering of the coarse focusing screw to drive the stage to coarsely adjust the focus includes: firstly controlling the coarse focusing screw to drive the stage to rise, and then controlling the coarse focusing screw to drive the stage to descend. The camera of the microscope collects an image every time the stage moves one step, and the collected image is input into the computer, and the computer intercepts the part of the image as the target object for focusing. The size of the 800X600 image is partially the target object. O...

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Abstract

The invention discloses a rapid automatic focusing method of a microscope, which comprises the following steps: firstly controlling a coarse focusing screw to drive an objective table to lift for coarse focus adjustment, and then controlling a fine focusing screw to drive the objective table to lift for fine focus adjustment, and the fine focus adjustment comprises the following steps: 1) taking a focusing distance interval obtained by coarse focus adjustment as a basis, a fine focusing screw is controlled to drive the objective table to ascend and descend in the focusing distance interval, images are collected, the definition of the target object is compared through a Tenenggrad gradient function, the clearest image is selected, and the corresponding optimal position of the objective table is obtained according to the clearest image; 2) verifying the optimal position of the objective table obtained in the step 1); and 3) controlling the fine focusing screw to drive the objective table to move to the optimal position of the objective table from the final staying position. According to the invention, the focusing speed and the focusing precision can be ensured, the microscope focusing can be quickly completed under a high magnification, and the focusing time can be greatly saved.

Description

technical field [0001] The invention relates to the technical field of microscopes, in particular to an automatic focusing method for a microscope. Background technique [0002] When using a microscope to observe the image on the glass slide, it is necessary to adjust the focal length of the microscope to obtain a clear picture. Due to manufacturing and matching precision problems of the existing coarse quasi-focus helix and fine quasi-focus helix that drive the microscope stage up and down, there is a gap between the actual rising or falling distance of the stage and the preset lifting distance when it drives the stage up and down. Deviations, which lead to the failure of the existing microscope automatic focusing method to focus under high-magnification lenses, and manual adjustments are often required to obtain the required clear images. Contents of the invention [0003] In view of this, the purpose of the present invention is to provide a rapid auto-focus method for ...

Claims

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Application Information

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IPC IPC(8): G02B21/34G02B21/32G02B21/24
CPCG02B21/34G02B21/32G02B21/241
Inventor 任松曾利刚朱倩雯
Owner 重庆切克威科技有限公司
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