The invention discloses a flexible and high-precision secondary mirror assembly focusing mechanism, which solves the problems of complex structure, poor focusing precision, easy stagnation in the focusing process, and complicated processing and assembly of the focusing mechanism in the traditional focusing method. The focusing mechanism includes: a base frame, a fixed ring, a flexible support guide mechanism, a detected ring plate, a piezoelectric ceramic actuator, a flexible amplification mechanism and a measurement feedback sensor; the base frame, a fixed ring, a detected ring plate, a flexible The supporting and guiding mechanisms are installed in sequence, and the focused secondary mirror assembly is installed on the flexible supporting and guiding mechanism. There are multiple piezoelectric ceramic actuators and flexible amplifying mechanisms, and they cooperate one by one to form multiple driving mechanisms for providing the focusing amount; A plurality of driving mechanisms are installed between the base frame and the supporting guide mechanism, and the measurement feedback sensor is installed on the base frame and used to measure the displacement of the focusing secondary mirror assembly.