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Fast Autofocus Methods for Microscopes

An auto-focus and microscope technology, applied in the field of microscopes, can solve problems such as inability to focus, and achieve the effects of saving focusing time, ensuring focusing speed, and ensuring focusing accuracy

Active Publication Date: 2022-07-29
重庆切克威科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of this, the purpose of the present invention is to provide a rapid auto-focusing method for a microscope to solve the technical problem that the existing microscope auto-focusing method cannot focus under a high-magnification lens and needs manual adjustment to complete the focusing

Method used

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  • Fast Autofocus Methods for Microscopes
  • Fast Autofocus Methods for Microscopes
  • Fast Autofocus Methods for Microscopes

Examples

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with the examples.

[0023] The fast automatic focusing method of the microscope in this embodiment includes firstly controlling the coarse focal-focus screw to drive the stage to move up and down to adjust the focal length, and then to control the fine focal-focus screw to drive the stage to move up and down to finely adjust the focal length.

[0024] Controlling the lifting and lowering of the coarse focusing screw to drive the stage to coarsely adjust the focus includes: firstly controlling the coarse focusing screw to drive the stage to rise, and then controlling the coarse focusing screw to drive the stage to descend. The camera of the microscope collects an image every time the stage moves one step, and the collected image is input into the computer, and the computer intercepts the part of the image as the target object for focusing. The size of the 800X600 image is partially the target object. O...

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Abstract

The invention discloses a fast automatic focusing method of a microscope, which comprises the steps of first controlling a coarse focal-focus screw to drive a stage to move up and down to roughly adjust the focal length, and then to control the fine focal-focus screw to drive the stage to move up and down to finely adjust the focal length. The fine focal length adjustment includes steps : 1) Based on the focusing distance interval obtained by coarsely adjusting the focal length, control the fine focus screw drive stage to first rise and then descend within the focusing distance interval and collect images, and use the Tenengrad gradient function to compare the clarity of the target object And select the clearest image, and obtain the corresponding optimal position of the stage according to the clearest image; 2) Verify the optimal position of the stage obtained in step 1); 3) Control the fine focus screw to drive the stage from the end. The stopped position is moved to the optimal position of the stage. The invention can not only ensure the focusing speed, but also ensure the focusing accuracy, and can also quickly complete the focusing of the microscope under high magnification, which can greatly save the focusing time.

Description

technical field [0001] The invention relates to the technical field of microscopes, in particular to an automatic focusing method of a microscope. Background technique [0002] When using a microscope to observe the image on the slide, it is necessary to adjust the focus of the microscope to obtain a clear picture. The existing coarse focus screw and fine focus screw that drive the microscope stage up and down, due to the problem of manufacturing and matching accuracy, when the stage is driven to lift, the actual lifting or lowering distance of the stage is different from the preset lifting distance. Deviation, which causes the existing microscope auto-focusing method to fail to focus under high magnification, and often requires manual manual adjustment to obtain the required clear image. SUMMARY OF THE INVENTION [0003] In view of this, the purpose of the present invention is to provide a fast automatic focusing method of a microscope, so as to solve the technical probl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/34G02B21/32G02B21/24
CPCG02B21/34G02B21/32G02B21/241
Inventor 任松曾利刚朱倩雯
Owner 重庆切克威科技有限公司
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