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Fixing device and construction method for long-time-interval real-time two-photon calcium imaging model of brain of sober free-activity mouse

A fixation device and two-photon technology, which is applied to the equipment, optics, and optical components of restraining animals, can solve the problems of expensive fixation devices, large range of head and neck movement, and small mouse skull volume, and achieve strong, reliable and stable , Observe the effect of regional stability and good tissue compatibility

Pending Publication Date: 2022-04-08
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a fixation device and a construction method suitable for a long-term real-time two-photon deep calcium imaging model of conscious and free-moving mouse brains, so as to solve the problem that the mouse brain is surrounded by the bone structure of the skull in the above-mentioned background technology. Medium and mouse skulls are small in size, with a large range of head and neck movement, and it is difficult to record long-term stable imaging. Moreover, the existing fixation devices are expensive, complicated to operate, difficult to get started, poor in fixation stability, and small in observation area.

Method used

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  • Fixing device and construction method for long-time-interval real-time two-photon calcium imaging model of brain of sober free-activity mouse
  • Fixing device and construction method for long-time-interval real-time two-photon calcium imaging model of brain of sober free-activity mouse
  • Fixing device and construction method for long-time-interval real-time two-photon calcium imaging model of brain of sober free-activity mouse

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Effect test

Embodiment 1

[0028] Fixation device suitable for long-term real-time two-photon deep calcium imaging model of awake freely moving mouse brain, including two-photon microscope fixed stand assembly 1, two-photon water mirror carrier magnetic ring 2 and fixed titanium needle 3, two-photon The microscope fixed stand assembly 1 includes a stand 11, a first dovetail groove 12 and a connecting and fixing component 13, the stand 11 is provided with a first dovetail groove 12, and the first dovetail groove 12 is slidably connected with a connecting and fixing component 13; The assembly 13 is composed of a base track slider 131, a first universal adjustment ball 132, a base connecting rod 133, a skull fixing pile connecting rod 134, a base connecting socket 135, a second universal adjusting ball 136, a nesting connection part 137, a fixing pile 138, a protrusion 139, a limit hole 1310 and a groove 1311, the base track slider 131 is slidably connected in the first dovetail groove 12; the base track sl...

Embodiment 2

[0036] A fixation device suitable for a long-term real-time two-photon deep calcium imaging model of a conscious and freely moving mouse brain, including a fixation titanium needle 3 and a stereotaxic clamp assembly 4, and the stereotaxic clamp assembly 4 includes a locator clamp base 41, The base fixing bolt 42, the second dovetail groove 43 and the connection and fixing assembly 13, the base fixing bolt 42 are arranged symmetrically on the locator clamp base 41, and the second dovetail groove 43 is provided on one side of the base fixing bolt 42, and the second dovetail groove 43 The upper slide is connected with a connection and fixing assembly 13; the connection and fixing assembly 13 is adjusted by the base track slider 131, the first universal adjustment ball 132, the base connecting rod 133, the skull fixing pile connecting rod 134, the base connecting socket 135, and the second universal adjustment. Ball 136, fixed pile nesting connection part 137, fixed pile 138, fixed...

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Abstract

The invention discloses a fixing device and a construction method suitable for a long-time-interval real-time two-photon calcium imaging model of the brain of a waking freely-moving mouse, the fixing device comprises a two-photon microscope fixing rack assembly, and the construction method comprises the following steps: step 1, fixing the head of the mouse; 2, dental cement pouring; step 3, establishing an observation area; and 4, mounting the magnetic suction ring. A two-photon water mirror loader magnetic ring is attached to the observation window part and is connected with the movable two-photon microscope sober mouse imaging auxiliary device, so that a water mirror lens can be borne and the lens can be moved and observed conveniently during later two-photon microscopic imaging, and the detachable magnetic device is convenient for long-time repeated observation; in combination with a matched novel movable two-photon microscope imaging auxiliary device, light can be shielded, and an experimenter can give various condition stimulations to an experimental mouse under a visual condition.

Description

technical field [0001] The invention relates to the technical field of establishing a real-time two-photon deep calcium imaging model of the mouse brain under waking and free activities, in particular to a fixture and a construction method suitable for a long-term real-time two-photon deep calcium imaging model of the waking and free activity mouse brain. Background technique [0002] With the development of technologies such as two-photon and ex vivo calcium imaging, people have a deeper understanding of the structure and function of neural networks in the mouse brain, but so far, the level of neural circuits in the free-moving brain of conscious mice There are few reports on calcium imaging research, because the mouse brain is wrapped in the bony structure of the skull, the mouse skull is small, the range of head and neck movement is large, long-term stable imaging records are difficult, and the existing fixation devices are expensive , complex operation, difficult to get ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00A61D3/00G02B21/26G02B21/36
Inventor 褚文广罗层解柔刚丁慧刘万能
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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