Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for determination of trimethylamine oxide, preparation method and application thereof

A trimethylamine oxide and kit technology, applied in the field of detection of endogenous biomarkers, can solve the inconsistency between batches of mixed matrix, the difficulty of applying in vitro diagnostic kits to industrialization, the source of mixed human blood matrix and ethical restrictions and other problems, to achieve the effect of excellent acceleration stability, precision and accuracy

Active Publication Date: 2022-07-15
长沙都正医学检验有限责任公司
View PDF14 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this approach ensures the “original taste” of the matrix, it ignores the fact that there are significant differences in TMAO content among samples, and the fact that mixed matrixes also have batch-to-batch inconsistencies, coupled with the fact that the source of mixed human blood matrix and ethics are restricted. Many limitations, so it is difficult to apply to the industrialization of the development of in vitro diagnostic kits (IVD)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for determination of trimethylamine oxide, preparation method and application thereof
  • Kit for determination of trimethylamine oxide, preparation method and application thereof
  • Kit for determination of trimethylamine oxide, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0063] The preparation of the above-mentioned kit provided by the present invention may include the following steps:

[0064] The trimethylamine oxide standard solution of known concentration is diluted with matrix solution to obtain the series of trimethylamine oxide calibrator working solutions with concentration gradient; wherein, the concentration of the known concentration trimethylamine oxide standard solution can be, for example, about 1 mg / mL, which is subject to actual weighing and preparation; the known concentration of trimethylamine oxide standard solution can also be called trimethylamine oxide calibrator stock solution;

[0065] The known concentration of the deuterated trimethylamine oxide standard solution is diluted with the matrix solution to obtain the deuterated trimethylamine oxide internal standard working solution of the predetermined concentration; wherein, the concentration of the known concentration of the deuterated trimethylamine oxide standard solu...

Embodiment 1

[0093] 1.1. Prepare the kit according to the following steps:

[0094] 1) Weighing: Weigh TMAO-d9 standard product, BSA and TMAO standard product respectively.

[0095] 2) Preparation of stock solution: The TMAO standard was prepared with a 50% (volume) methanol aqueous solution into a calibrator stock solution of trimethylamine oxide with a concentration of 1 mg / mL and a quality control solution of trimethylamine oxide with a concentration of 1 mg / mL, respectively. The -d9 standard was prepared into a stock solution of deuterated trimethylamine oxide with a concentration of 1 mg / mL in 50% (volume) methanol aqueous solution.

[0096] 3) Matrix aqueous solution: The matrix BSA was prepared into a 5% matrix solution with 10 mM PBS at pH 7.4.

[0097] 4) Dilute the trimethylamine oxide calibrator stock solution, trimethylamine oxide quality control stock solution, and deuterated trimethylamine oxide internal standard stock solution with the matrix aqueous solution to obtain a se...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides a kit for measuring trimethylamine oxide, a preparation method and application thereof, the kit provided by the invention can effectively solve the current situation of poor stability of the kit prepared by the solution process, and can realize the freezing of the internal standard. Dry and long term stability. The kit includes a series of trimethylamine oxide calibrators and deuterated trimethylamine oxide internal standards; the series of trimethylamine oxide calibrators are lyophilized products of a series of trimethylamine oxide calibrator working solutions with a concentration gradient, and the series of trimethylamine oxide calibrators are lyophilized. The trimethylamine oxide calibrator working solution is a trimethylamine oxide calibrator working solution with a concentration gradient obtained by diluting the known concentration of the trimethylamine oxide standard solution with the matrix solution; the deuterated trimethylamine oxide internal standard is known The lyophilized product of the deuterated trimethylamine oxide internal standard working solution with a predetermined concentration obtained by diluting the deuterated trimethylamine oxide standard solution with the concentration of the matrix solution; the matrix in the matrix solution is serum albumin.

Description

technical field [0001] The invention relates to the technical field of detection of endogenous biological markers, in particular to a kit for determining trimethylamine oxide, a preparation method and application thereof. Background technique [0002] As an endogenous biomarker, trimethylamine oxide (TMAO), like other biomarkers, needs to resolve matrix interference first to ensure quantitative accuracy. Therefore, when choosing a suitable matrix, developers often take 2 main ways: [0003] 1) Subtract the zero-concentration blank blood matrix, that is, select mixed blood as the matrix, subtract the calibrators and quality control substances of different concentrations from the zero-concentration mixed blood matrix, and remove the TMAO interference inherent in the mixed blood matrix. Curve fitting and assay of samples. Although this approach ensures the "original taste" of the matrix, it ignores the fact that there are significant differences in TMAO content between sample...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72G01N30/04
CPCG01N30/02G01N30/06G01N30/34G01N30/72G01N2030/045
Inventor 侯利平王婷朱永瑞阳艳李超鹏李晓晖欧阳冬生
Owner 长沙都正医学检验有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products