Kit for determination of trimethylamine oxide, preparation method and application thereof
A trimethylamine oxide and kit technology, applied in the field of detection of endogenous biomarkers, can solve the inconsistency between batches of mixed matrix, the difficulty of applying in vitro diagnostic kits to industrialization, the source of mixed human blood matrix and ethical restrictions and other problems, to achieve the effect of excellent acceleration stability, precision and accuracy
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[0063] The preparation of the above-mentioned kit provided by the present invention may include the following steps:
[0064] The trimethylamine oxide standard solution of known concentration is diluted with matrix solution to obtain the series of trimethylamine oxide calibrator working solutions with concentration gradient; wherein, the concentration of the known concentration trimethylamine oxide standard solution can be, for example, about 1 mg / mL, which is subject to actual weighing and preparation; the known concentration of trimethylamine oxide standard solution can also be called trimethylamine oxide calibrator stock solution;
[0065] The known concentration of the deuterated trimethylamine oxide standard solution is diluted with the matrix solution to obtain the deuterated trimethylamine oxide internal standard working solution of the predetermined concentration; wherein, the concentration of the known concentration of the deuterated trimethylamine oxide standard solu...
Embodiment 1
[0093] 1.1. Prepare the kit according to the following steps:
[0094] 1) Weighing: Weigh TMAO-d9 standard product, BSA and TMAO standard product respectively.
[0095] 2) Preparation of stock solution: The TMAO standard was prepared with a 50% (volume) methanol aqueous solution into a calibrator stock solution of trimethylamine oxide with a concentration of 1 mg / mL and a quality control solution of trimethylamine oxide with a concentration of 1 mg / mL, respectively. The -d9 standard was prepared into a stock solution of deuterated trimethylamine oxide with a concentration of 1 mg / mL in 50% (volume) methanol aqueous solution.
[0096] 3) Matrix aqueous solution: The matrix BSA was prepared into a 5% matrix solution with 10 mM PBS at pH 7.4.
[0097] 4) Dilute the trimethylamine oxide calibrator stock solution, trimethylamine oxide quality control stock solution, and deuterated trimethylamine oxide internal standard stock solution with the matrix aqueous solution to obtain a se...
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