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Application of hericium erinaceus A in preparation of weight-losing and lipid-lowering medicine

A technology of ericin and medicine is applied in the application field of ericin A in the preparation of weight-loss and lipid-lowering medicine, and achieves the effects of inhibiting the growth of inguinal fat and inhibiting the rise of blood lipids

Pending Publication Date: 2022-04-29
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the high nutritional value of Hericium erinaceus, it has become people's daily food and drink, but Erinacine A (Erinacine A), a compound in Hericium erinaceus, has not been reported for weight loss and lipid-lowering.

Method used

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  • Application of hericium erinaceus A in preparation of weight-losing and lipid-lowering medicine
  • Application of hericium erinaceus A in preparation of weight-losing and lipid-lowering medicine
  • Application of hericium erinaceus A in preparation of weight-losing and lipid-lowering medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Erinacine A reduces the differentiation of preadipocyte 3T3-L1

[0016] experimental method:

[0017] 1. CCK-8 detects the cytotoxicity of Erinacine A to 3T3-L1

[0018] In order to study the weight loss and lipid-lowering functions of Erinacine A in this example, the cytotoxicity of Erinacine A to 3T3-L1 was detected by CCK-8 experiment. Briefly, 3T3-L1 cells were seeded in 96-well plates at 37 o C. 5% CO 2 Cell culture incubator overnight. Then, 10 microliters of Erinacine A of various concentrations were added to the cultured cells and incubated for 24 hours. Add 10 microliters of CCK-8 solution to each well (to avoid air bubbles affecting the OD value), place the culture plate in the incubator for 2.5 hours, and then use a microplate reader to measure the absorbance at 450 nm (reference wavelength for wavelengths of 600 nm or above).

[0019] 2. A model for inducing 3T3-L1 differentiation into adipocytes

[0020] 3T3-L1 cells were purchased from the National ...

Embodiment 2

[0028] Erinacine A can inhibit the growth of inguinal fat and the increase of blood lipid in HFD mice

[0029] experimental method:

[0030] 1. Count the daily food intake of each mouse

[0031] Purchase mice fed with HFD for 10 w, that is, HFD mice (where HFD mice refer to 6-week-old C57BL / 6 mice that have been continuously fed with HFD diet for 10 w), and intragastrically administer Erinacine A every day (2 g / kg body weight), while continuing to feed with normal feed for 2 weeks. The daily food intake of each mouse was statistically analyzed. Two HFD mice per group.

[0032] 2. Weighing the inguinal fat and mouse body weight of HFD mice

[0033] Buy HFD and feed the mice after 10 w. Erinacine A (2 g / kg body weight) was intragastrically administered every day, while continuing to feed with normal feed for 2 weeks. The inguinal fat and body weight of mice in each group were weighed, and the ratio of the two was calculated.

[0034] 3. Detect the blood lipid content of H...

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Abstract

The invention discloses application of erinacine A in preparation of weight-losing and lipid-lowering medicines, and belongs to the technical field of biological medicines. Experiments show that Erinacine A can significantly inhibit mouse obesity caused by high fat diet; meanwhile, the Erinacine A can significantly inhibit differentiation of preadipocytes and reduce mouse groin fat accumulation caused by high fat diet.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of erinacein A in the preparation of weight-loss and lipid-lowering drugs. Background technique [0002] With the improvement of living standards, people's diet is becoming richer and richer, resulting in more and more people becoming obese. Obese people are often more prone to disorders of liver lipid metabolism, diabetes, cardiovascular sclerosis, and urinary system diseases. However, the medicines and technical means for preventing and treating obesity still need to be improved. Therefore, research and development of pharmaceutical preparations for preventing and treating obesity has important application value. [0003] Due to the high nutritional value of Hericium erinaceus, it has become people's daily catering food, but Erinacine A (Erinacine A), a compound in Hericium erinaceus, has not been reported for weight loss and lipid-lowering. ...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P3/04A61P3/06
CPCA61K31/704A61P3/04A61P3/06
Inventor 陈添兴
Owner NANTONG UNIVERSITY