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Method and microscope having correction device for correcting color difference induced imaging errors

A technology of imaging error and chromatic aberration, applied in the field of imaging error induced by chromatic aberration, and can solve problems such as incomparability

Pending Publication Date: 2022-04-29
インスティテュートフュール ナノフォトニクゲッティンゲンイーヴィー
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the corresponding maxima or minima of an indicator combining image brightness and image sharpness have only local validity (Aussagekraft) and cannot be compared with other maxima or minima in other regions of the sample

Method used

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  • Method and microscope having correction device for correcting color difference induced imaging errors
  • Method and microscope having correction device for correcting color difference induced imaging errors
  • Method and microscope having correction device for correcting color difference induced imaging errors

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Embodiment Construction

[0070] exist figure 1 The laser scanning microscope 1 according to the invention shown in is based on a STED microscope and accordingly has, in addition to a first light source 2 for excitation light 3 , a second light source 4 , which is light in the form of stimulating light 6 5, this light 5 is used for the excitation caused by the excitation light 3 which is again eliminated. Each of the two light sources 2 and 4 comprises a laser 7 or 8, a polarization maintaining fiber 9 or 10 and collimation optics 11 or 12 for the excitation light 3 or stimulation light 6 emerging from the fiber 9 or 10 . An SLM 13 for wavefront shaping is also arranged in the beam path of the stimulation light 6 so that the stimulation light 6 forms a light intensity distribution with a central intensity minimum in the focal region of the lens 14 . Stimulation light 6 is combined with excitation light 3 by means of dichroic beam splitter 16 downstream of SLM 13 after further optics 15 . Via a defor...

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Abstract

In order to correct imaging errors induced by color differences of an optical system having a lens (14) and adaptive optics (18), the light (5) and the sample (20) are selected in such a way that the light (5) reduces a measurement signal (28) from the sample (20) when acting on the sample (20), the relative change of the measurement signal (28) depending on the intensity of the light (5). A measurement signal (28) from a focal region of the optical system in the sample (20) is detected over a first and a second time period (38, 37) following the first time period in order to determine a first and a second measurement value. The light (5) is focused into the focal region by means of the optical system over a third period of time (39) that overlaps the first and / or second period of time. A metric value for the relative change of the measurement signal (28) is determined from the first and second measurement values and is used as an indicator to be optimized in the actuation of the adaptive optics (18).

Description

technical field [0001] The invention relates to a method for correcting chromatic aberration-induced imaging errors of an optical system having a lens and adaptive optics in the beam path passing through the lens. Furthermore, the present invention relates to a laser scanning microscope having a first light source for excitation light, a second light source for excitation light, a lens, and a correction device for correcting imaging errors induced by chromatic aberration, the The correction device includes adaptive optics in the beam path through the lens. [0002] On the one hand, chromatic aberration-induced imaging errors can be attributed to the optical system itself, for example, if the optical system is not sufficiently achromatic. On the other hand, the sample area located in front of the focal area of ​​the optical system can also cause potential aberrations. [0003] Imaging errors to be corrected induced by chromatic aberrations of the optical system play a role no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/01G02B21/06G02B21/00
CPCG01N21/6458G01N21/6402G01N21/6408G01N21/6428G01N21/01G02B21/06G02B21/002G01N2021/6439G02B21/0076G02B21/0084G02B21/006G02B21/241G02B26/06G02B27/0025G02B27/58G02B2207/114G02B7/40
Inventor A·艾格纳C·盖斯勒F·罗卡
Owner インスティテュートフュール ナノフォトニクゲッティンゲンイーヴィー