Gamma-aminobutyric acid-containing rice culture solution, application of gamma-aminobutyric acid-containing rice culture solution in nitrogen stress resistance of rice and rice culture method
A technology of aminobutyric acid and a culture method, which is applied in the application of rice resistance to nitrogen stress, in the field of rice culture, can solve the problems of difficulty in improving the growth state of rice biomass tiller buds and the like
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Embodiment 1
[0049] Example 1: Effects of hydroponics GABA treatment on rice growth and development under different nitrogen concentrations
[0050] Rice soaking: soak the rice seeds with 6 / 1000 diluted nitric acid for 8 hours. Among them, the rice seeds were ZH11 rice seeds (the seeds were all from the Laboratory of Plant Hormone and Nutrient Molecular Regulation, Guizhou University).
[0051] Cultivation of rice seeds: After soaking, rinse the seeds 3-5 times, divide them into petri dishes, add appropriate purified water, and put them into an incubator at 37°C for cultivation, and change the water regularly 2-3 times a day.
[0052] Hydroponics: When the rice was uniformly cultivated to the 3-leaf stage, 12L of ordinary rice culture medium was added to the culture pots, and then the rice was moved to the culture pots for cultivation. 18 culture pots were set up, and 6 culture pots were set as the low nitrogen group. 5 culture pots were in the medium nitrogen group, and 7 culture pots we...
Embodiment 2
[0058] Example 2: Effect of GABA on chlorophyll content of rice leaves
[0059] The materials of rice in this example are all from the above-mentioned culture materials, and because the difference in leaf color phenotype between the low nitrogen group and the medium nitrogen group is not obvious, this example mainly explores the chlorophyll content of the high nitrogen group. The measurement method of chlorophyll content in rice leaves was carried out with reference to the literature (Nur Kaimer Mulati, Yang Yajie, Parhati Abdukrimu, et al. Comparison of methods for determination of chlorophyll content in wheat[J]. Jiangsu Agricultural Science, 2021, 49(09):156-159.).
[0060] Material of rice leaves: From the above-mentioned high nitrogen group rice seedlings, the flag leaves of every three rice plants were taken for each treatment, and the leaves except the veins were cut into pieces and mixed evenly. Weigh 2-3g of leaf fragments for each group of treatments, put them in a ...
Embodiment 3
[0069] Example 3: Effects of hydroponic application of exogenous GABA and foliar spray on rice growth and development
[0070] See Example 1 for the process of soaking rice seeds, cultivating rice seeds and hydroponic cultivation. In the hydroponic process of this embodiment, 9 cultivation pots are set up, 3 cultivation pots are respectively set as the low nitrogen group, and 3 cultivation pots are The medium nitrogen group and the three culture pots were the high nitrogen group.
[0071] The GABA treatment conditions are as follows: After 3 days of hydroponics, the seedlings have basically adapted to the culture box. In order to reduce the workload and facilitate the follow-up work, GABA mother liquor was prepared, and the concentration of the mother liquor was 1 mol / L. First, 0ml (control group), 0ml (foliar spray), and 36ml (hydroponic application) GABA stock solution were added to 12L of low-nitrogen nutrient solution in 3 culture pots of low-nitrogen group respectively, ...
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