Method for improving observation effect of aquatic animal tissues

A technique for slicing aquatic animals and tissues, which is applied in the preparation, sampling, and instrumentation of test samples. It can solve the problems of affecting observation effects, disordered collagen fiber structure, and difficulty in obtaining tissue structures, so as to reduce changes in tissue structures and facilitate operation. Simple, time-saving and cost-effective

Pending Publication Date: 2022-05-27
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional paraffin section preparation process, the sample is usually directly fixed in 10% paraformaldehyde solution after sampling, and then a series of steps such as dehydration, transparency, wax immersion, embedding, and sectioning are performed. During the sampling or processing of live aquatic animals, the muscle fibers of aquatic animals will shrink and then assemble, resulting in disordered collagen fiber structure, which will affect the observation effect and make it difficult to obtain accurate, ideal and clear tissue structures.

Method used

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  • Method for improving observation effect of aquatic animal tissues
  • Method for improving observation effect of aquatic animal tissues
  • Method for improving observation effect of aquatic animal tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation of paraffin sections

[0031] S1, sampling and balancing: get fresh sea cucumber and soak it in artificial seawater balance solution (3.5% sodium chloride solution) for equilibration treatment for 1h to obtain the balanced sea cucumber living body;

[0032] S2, anesthesia: soak the sea cucumber living body described in step S1 in ice-warm seawater anesthesia solution (containing 3.5% MgCl 2 In the artificial seawater balance solution), anesthesia treatment is carried out for 1h to obtain anesthetized sea cucumber living body;

[0033] S3. Cut into pieces: Take the live sea cucumber after anesthesia, cut it along the abdomen, remove the teeth and internal organs, clean it, put the body wall of the sea cucumber down, cut the abdomen longitudinally, cut the head and tail transversely, and flatten the back of the obtained sea cucumber. put, cut into 0.5cm x 0.5cm x 0.5cm sea cucumber body wall sample blocks;

[0034] S4, fixation: immerse the sea cu...

Embodiment 2

[0045] Example 2 Preparation of frozen sections

[0046] S1, sampling and balancing: get fresh sea cucumber and soak it in artificial seawater balance solution (3.5% sodium chloride solution) for equilibration treatment for 1h to obtain the balanced sea cucumber living body;

[0047] S2, anesthesia: soak the sea cucumber living body described in step S1 in ice-warm seawater anesthesia solution (containing 3.5% MgCl 2 In the artificial seawater balance solution), anesthesia treatment is carried out for 1h to obtain anesthetized sea cucumber living body;

[0048] S3. Cut into pieces: Take the live sea cucumber after anesthesia, cut it along the abdomen, remove the teeth and internal organs, clean it, put the body wall of the sea cucumber down, cut the abdomen longitudinally, cut the head and tail transversely, and flatten the back of the obtained sea cucumber. put, cut into 0.5cm x 0.5cm x 0.5cm sea cucumber body wall sample blocks;

[0049] S4, cut the sea cucumber body wall ...

Embodiment 3

[0054] S1, sampling and balancing: get fresh sea cucumber and soak it in artificial seawater balance solution (3.5% sodium chloride solution) for equilibration treatment for 1h to obtain the balanced sea cucumber living body;

[0055] S2, anesthesia: soak the sea cucumber living body described in step S1 in ice-warm seawater anesthesia solution (containing 3.5% MgCl 2 In the artificial seawater balance solution), anesthesia treatment is carried out for 1h to obtain anesthetized sea cucumber living body;

[0056] S3. Cut into pieces: Take the live sea cucumber after anesthesia, cut it along the abdomen, remove the teeth and internal organs, clean it, put the body wall of the sea cucumber down, cut the abdomen longitudinally, cut the head and tail transversely, and flatten the back of the obtained sea cucumber. put, cut into 0.5cm x 0.5cm x 0.5cm sea cucumber body wall sample blocks;

[0057] S4. The sea cucumber body wall sample block is placed in a 2.5% glutaraldehyde solutio...

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Abstract

The invention discloses a method for improving the observation effect of aquatic animal tissues, and belongs to the field of tissue observation. According to the method, the aquatic animals are subjected to balance and anesthesia pretreatment to obtain samples, and then the samples are prepared into the slices, so that tissue structure change caused by stress reaction of the aquatic animals can be effectively reduced, more accurate tissue structure change of the aquatic animals can be obtained, and tissue slices with clear structures can be obtained; the scientific development of the aquatic animal research field is promoted. The sea cucumber sample obtained after balancing and anesthesia pretreatment can be directly used for preparing frozen slices, so that the time cost is saved to a great extent. The method provided by the invention is simple and convenient to operate, remarkable in effect, low in cost and convenient to popularize.

Description

technical field [0001] The invention relates to a method for improving the observation effect of aquatic animal tissue, belonging to the field of tissue observation. Background technique [0002] Both sea cucumber and sea urchin belong to the phylum echinoderms. Sea cucumber is high in protein, low in fat and rich in essential amino acids, vitamins, essential fatty acids and macro and trace elements; sea urchin is rich in eicosenoic acid, and its extract Bonelin Has the effect of inhibiting the growth of cancer cells. At present, the high nutritional value of sea cucumber is recognized by more and more people, so the research on its processing characteristics is increasing. The body wall of sea cucumber is mainly composed of collagen tissue, and there are glycosaminoglycan sugar bridge structures between collagen tissues. The degradation of these structures under different processing conditions will lead to changes in the body wall tissue structure of sea cucumber, and its ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N1/28G01N1/30
CPCG01N21/84G01N1/286G01N1/30G01N2001/2873Y02A40/81
Inventor 董秀萍张娜娜韦时彪穆云奎秦磊姜鹏飞王书晨李子金
Owner DALIAN POLYTECHNIC UNIVERSITY
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