Preparation method and application of bridged cyclodextrin/chitosan bifunctional chiral monolithic column
A technology of cyclodextrin and chitosan, which is applied in the field of analytical chemistry, can solve the problems of insufficient development and achieve the effects of improving chiral separation efficiency and separation effect, increasing the recognition area, and improving separation efficiency
Active Publication Date: 2022-07-01
FUZHOU UNIV
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Problems solved by technology
[0006]
Aiming at the problem that the current development of bridging cyclodextrin bridging agent is not extensive enough, and it is not used in the field of chitosan "synergy" with chitosan, the present invention provides a bridging cyclodextrin / chitosan Preparation method of bifunctional chiral monolithic column and its application in enantiomer separation
Method used
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Embodiment 1
[0033] A preparation method of a bridged cyclodextrin / chitosan bifunctional chiral monolithic column, the specific steps are:
[0034] (1) Accurately weigh 1 mg of EBA, 13.69 mg of SH-β-CD, 1 mg of AIBA and 225 mg of deionized water into a centrifuge tube, vortex for 1 min to fully dissolve, sonicate for 30 min, under the condition of 60 ℃ water bath After 6 h of polymerization, bridged cyclodextrins were generated.
[0035] (2) 2.5 mg CS (viscosity 3OH to remove unreacted monomers to give column C.
Embodiment 2
[0037] In step (2), 1.5 mg of chitosan was added, and other steps were as in Example 1 to obtain column A.
Embodiment 3
[0039] In step (2), 2.0 mg of chitosan was added, and other steps were as in Example 1 to obtain column B.
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Abstract
The invention discloses a preparation method and application of a bridged cyclodextrin / chitosan bifunctional chiral monolithic column, and belongs to the field of analytical chemistry. The monolithic column is rapidly prepared within 30 minutes by constructing a chiral environment through bridging cyclodextrin and chitosan double monomers and adopting a one-pot thermal polycondensation method with urea and formaldehyde. The preparation method disclosed by the invention is simple and rapid in reaction, and the bridging cyclodextrin and chitosan are used as chiral functional monomers to establish a co-functionalization system with a'synergistic effect 'of the double chiral selectors, so that the framework of the monolithic column has the double chiral selectors at the same time, the number of chiral centers and the separation effect are improved, and the separation efficiency is improved. The chiral separation of the N-carbobenzoxy-DL-asparagine, the DL-tyrosine and the DL-phenylalanine is realized by virtue of the electrochromatography.
Description
technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a preparation method and application of a bridged cyclodextrin / chitosan bifunctional chiral monolithic column. Background technique [0002] Chiral isomers are two compounds with the same molecular formula but different spatial structures. They are widely used in medicine, clinical, pathology and other fields. Although their physical and chemical properties are almost the same, different enantiomers have different effects on pharmacological activity and metabolic effects. , toxicity and other aspects are very different, and even have opposite effects. Therefore, it is of great significance to realize the separation of chiral compounds. In the past few decades, chiral monolithic columns have been favored by researchers in the field of enantiomeric separation due to their good mass transfer performance, low back pressure and high separation efficiency. However, the preparation ...
Claims
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IPC IPC(8): B01J20/26B01J20/29B01J20/30B01D15/38
CPCB01J20/24B01J20/262B01J20/29B01D15/3833
Inventor 吕海霞刘玥曾宇
Owner FUZHOU UNIV