Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Medicine for atherosclerosis

A technique for atherosclerosis and coronary arteries, applied in the medical field to achieve the effect of reducing apoptosis

Active Publication Date: 2022-07-15
青岛山大齐鲁医院(山东大学齐鲁医院(青岛))
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the application of talcoside in atherosclerosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Medicine for atherosclerosis
  • Medicine for atherosclerosis
  • Medicine for atherosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The role of salivaricin in the growth inhibition of HCAEC cells (human coronary artery endothelial cells) induced by oxidized low-density lipoprotein

[0030] (1) The HCAEC cells with a good growth state and a growth density of 80% were collected into a centrifuge tube according to the method of cell passage, and the cells were resuspended in complete medium;

[0031] (2) Take 10ul of the cell suspension for counting using a hemocytometer, and then dilute the cell suspension to a density of 3 × 10 according to the end result of the cells. 4 pcs / ML;

[0032] (3) Add 100ul of suspension to each well of the 96-well plate, shake gently to mix, and then add PBS to the surrounding wells where the cells are seeded to prevent edge effects caused by water evaporation;

[0033] (4) After 24 hours, the medium in the 96-well plate was removed, and 100ul serum-free medium containing 0.1% bovine serum albumin was added to each well to make the cells starved and adjusted to the same ...

Embodiment 2

[0037] (1) The HCAEC cells were seeded in 6-well medium plates, and when the cell density reached 85%, the control group was cultured with complete medium; the experimental group a was treated with 0ug / ml phloxacin for 24 hours, and then treated with 100mg / L The complete medium of ox-LDL was cultured for 24 hours; after the experimental group b was treated with 25ug / ml saccharin for 24 hours, the complete medium containing 100mg / L ox-LDL was cultured for 24 hours, and each group was set with 3 replicate wells;

[0038] (2) After the treatment, remove the medium, wash the cells with PBS, add 100ul RIPA cell lysis solution to each well, and fully lyse for 30 minutes;

[0039] (3) After the lysis is completed, transfer the lysate to a centrifuge tube, then place the centrifuge tube in a low-temperature high-speed centrifuge, centrifuge at 12,000g / min for 18 minutes at 4°C, take out the centrifuge tube, and transfer the supernatant to a new centrifuge. in the tube;

[0040] (4) T...

Embodiment 3

[0043] (1) Put the prepared gel into the vertical electrophoresis apparatus, and add the electrophoresis buffer to the designated scale line of the electrophoresis tank;

[0044] (2) Add 20ug protein sample to the gel hole, and add protein marker to the first gel hole;

[0045] (3) Install the electrophoresis instrument correctly in the order of positive and negative electrodes. The electrophoresis condition of the concentrated gel is 80V for 30min, and then adjust the voltage to 120V until the electrophoresis band is at the bottom, and stop the electrophoresis;

[0046] (4) Soak the PVDF membrane in methanol, ultrapure water and membrane transfer buffer in sequence. At the same time, soak the sponge in the membrane transfer buffer for 30 minutes. After electrophoresis, take out the gel and follow the steps of sponge, PVDF membrane, gel , Put the sponges in order and put them in the semi-dry film transfer machine, transfer the film at 250mA for 90min;

[0047] (5) After the m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a medicine for atherosclerosis, and belongs to the technical field of medicine. The medicine comprises tracheloside and a pharmaceutically acceptable carrier. It is found that the tracheloside can effectively reduce the reduction of the survival activity of human coronary endothelial cells caused by ox-LDL, and can effectively reduce the protein expression increase of endothelial cell vascular adhesion molecules VCAM1 and intercellular adhesion molecules ICAM1 in the human coronary endothelial cells caused by ox-LDL. Meanwhile, cell apoptosis rise caused by ox-LDL can be effectively reduced. Therefore, the tracheloside is creatively used for preparing the medicine for treating the atherosclerosis.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a medicine for atherosclerosis. Background technique [0002] In recent years, the number of patients with cardiovascular disease in China has continued to increase. At present, cardiovascular disease has become the primary threat to death of urban and rural residents in China. Among them, atherosclerosis is a common arterial vascular disease, which is caused by the deposition of atheromatous plaques composed of lipids, cholesterol, calcium and other substances and surrounding cells on the blood vessel wall, resulting in vascular stenosis. Plaques can also rupture to form thrombus and have developed other ischemic diseases. [0003] Vascular endothelial cells play an important role in atherosclerosis. When vascular endothelial cells are damaged, they will make monocytes aggregate to early stage by up-regulating the expression of endothelial cell vascular adhesion mo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P9/10
CPCA61K31/7048A61P9/10
Inventor 孙慧王颖翠
Owner 青岛山大齐鲁医院(山东大学齐鲁医院(青岛))
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com