Novel coronavirus detection kit and preparation method thereof

A detection kit and coronavirus technology, applied in the field of medical testing, can solve the problems of low sensitivity of immune detection, and achieve the effects of strong specificity, low detection cost and high sensitivity

Pending Publication Date: 2022-07-19
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with conventional colloidal gold chromatography reagents, the detection sensitivity is increased by more than an order of magnitude, which solves the bottleneck technical problem of low immunological detection sensitivity, and makes up for the original congenital deficiency of immunodiagnostic products. It is suitable for primary medical institutions and public health emergency monitoring sites. Rapid Screening Diagnosis

Method used

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  • Novel coronavirus detection kit and preparation method thereof
  • Novel coronavirus detection kit and preparation method thereof
  • Novel coronavirus detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A detection kit for novel coronavirus

[0045] Among them, the structure of the immunochromatographic surface-enhanced Raman spectroscopy test strip for detecting the new coronavirus is as follows: figure 1 and figure 2 As shown, it includes sample pad 2, binding pad 3, nitrocellulose membrane 4, absorbent pad 5 and PVC backing 1.

[0046] A sample pad, a binding pad, a nitrocellulose membrane and a water-absorbing pad are successively adhered to the PVC backing, the binding pad and the nitrocellulose membrane are overlapped, and the nitrocellulose membrane and the water-absorbing pad are overlapped; The binding pad was coated with Wu-DTNB@Ag-Ab1 marker, and the nitrocellulose membrane was coated with a detection line T composed of the new crown N protein antibody 2 and a quality control line C composed of rabbit anti-mouse IgG, respectively.

[0047] The coating concentration of the new crown N protein antibody 2 is 0.5 mg / mL; the coating concentration of the rabbi...

Embodiment 2

[0051] A kind of preparation method of novel coronavirus detection kit

[0052] (1) Preparation of nano-silver particle solution: Weigh 0.018g of silver nitrate and dissolve it in 100mL of ultrapure water, place the prepared silver nitrate solution in an oil bath (130°C) and heat while stirring. When the solution is boiled, add 4 mL of 1% trisodium citrate solution, continue to heat for 1 hour, and then cool to 20-25° C. to obtain nano-silver particle solution (AgNPs);

[0053] (2) Surface modification of DTNB on silver nanoparticles: take 10 mL of the silver nanoparticles solution prepared in step (1), add 20 μL of 0.01 mol / L DTNB solution, stir and react at room temperature for 2 h, 12000 r / min, centrifuge for 10 min, and remove the supernatant solution, and the precipitation was suspended with 2 mL of ultrapure water to obtain the AgNPs-DTNB solution;

[0054] (3) Preparation of tungsten-wrapped AgNPs-DTNB core-shell structure: take 2 mL of the AgNPs-DTNB solution prepared...

Embodiment 3

[0061] Establishment of standard curve

[0062] Take the new crown recombinant N protein and dilute it with the sample extract to obtain concentrations of 1pg / mL, 10pg / mL, 100pg / mL, and 500pg / mL. After testing with the above test strips, it is detected by confocal laser Raman spectrometer. The characteristic peak intensity of the T-line Raman signal molecule DTNB is used to draw the standard curve, see image 3 .

[0063] technical effect

[0064] specific test

[0065] The pathogenic bacteria or recombinant proteins used for specific tests include SARS-N protein, MERS-N protein, and H1N1 virus isolates.

[0066] Dilute the concentration of the analyte to 100ng / mL, accurately draw 80 μL of the sample to be tested with a pipette, and drop it vertically and slowly into the sample hole of the test card. Start timing after adding the sample, and read the result 10 minutes after adding the sample. The test results are shown in Table 1:

[0067] Table 1 test strip specificity t...

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Abstract

The invention discloses a novel coronavirus detection kit and a preparation method thereof. Belongs to the technical field of medical examination. Comprising an immunochromatography surface enhanced Raman spectrum test strip for detecting the new crown virus and a sample extracting solution, and the test strip specifically comprises a Wu-DTNB (at) Ag-Ab1 solution uniformly coated on a combination pad, a new crown N protein antibody 2 coated on a nitrocellulose membrane to form a detection line, and rabbit anti-mouse IgG coated on the nitrocellulose membrane to form a quality control line. The test strip comprises a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a PVC backing, and the sample pad, the combination pad, the nitrocellulose membrane and the water absorption pad are sequentially and continuously adhered to the PVC backing. The kit has the advantages of strong specificity, high sensitivity, short detection time, on-site operation, low detection cost, simplicity and convenience in operation and the like.

Description

technical field [0001] The invention relates to the technical field of medical testing, and more particularly to a detection kit for a novel coronavirus and a preparation method thereof. Background technique [0002] As a diagnostic method, nucleic acid detection plays an important role in the clinical diagnosis of patients and the screening of suspected patients. However, due to the cumbersome operation, time-consuming, centralized inspection and other limitations, the suspected patients cannot be diagnosed and shunted in time, and the detection also has false negative defects. [0003] Immune detection is based on the principle of specific reaction between antibodies and antigens. It detects infection by detecting viral proteins (antigens) in the body, or antibodies specific to viral proteins in the body. It is widely used in HIV, hepatitis B, C Detection of major infectious diseases such as hepatitis. [0004] The new coronavirus detection is to realize the detection fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543G01N33/558G01N33/531B22F1/00B22F9/24B82Y30/00B82Y40/00
CPCG01N33/56983G01N33/54346G01N33/558G01N33/531B22F9/24B82Y40/00B82Y30/00G01N2333/165
Inventor 金涌陈艳邹明强薛强殷宏
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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