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Method of raising taxinol content in tazus chinensis

A technology of yew and paclitaxel, applied in botany equipment and methods, chemicals for biological control, animal repellent, etc., can solve the problems of paclitaxel research on plants and lack of regulation technology, etc., to reduce production costs , Improve economic efficiency and increase output

Inactive Publication Date: 2007-05-23
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, many studies are aimed at cell culture research, and there are few studies on paclitaxel metabolism in plants, and there is a lack of effective regulation technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: the treatment of yew in the greenhouse

[0012] The treatment materials are 4-year-old yew saplings cultivated in the greenhouse. Select the saplings with the same plant height and branch number, and spray 0.05mg / L coronatin on the foliage of the yew tree, repeating 3 times, every adjacent two The spraying time interval is 15 days. Among them, it is feasible to choose yew trees that are 3-5 years old in the greenhouse, the concentration of coronatin is 0.05-0.1mg / L, the spraying interval is 10-20 days, and the number of repeated spraying is within the range of 2-4 times. .

[0013] Determination of paclitaxel content after 2 weeks: take 5 plants for each repetition, rinse the root system with tap water first after sampling, then wash the whole plant with distilled water, divide the samples after absorbing the water with absorbent paper, and cut the lateral roots, stems, new leaves and old leaves respectively. Leaves, weighed 1g of the sample and added 5m...

Embodiment 2

[0017] Embodiment 2: wild yew processing

[0018] The treatment material is the 6-year-old yew propagated by cuttings in the field cultivation base, and the material with similar plant height is selected for treatment. In September, the concentration of 0.1mg / l coronatin is sprayed on the leaves of the yew tree, repeated 4 times, and each adjacent two The spraying time interval is 12 days. Among them, the field cutting yew trees are selected to be 5-10 years old, the spraying time is 8-10 months, the concentration of coronatin is 0.05-0.1mg / l, the number of spraying repetitions is 3-5 times, and the interval time is 10-15 days. feasible.

[0019] Determination of paclitaxel content after 2 weeks: Take 5 plants for each repetition, cut the stems and needles respectively, air-dry and crush them, pass through a 100-mesh sieve, weigh 1g of the mixed sample and put it into a 20ml test tube, add 10ml of methanol into an ultrasonic cleaner Sonicate for 4 hours, centrifuge and pour ...

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Abstract

A method for increasing the content of taxol in enqlish yew features that the solution of coronary bacteruim extract onto the stem and leaves of young enqlish yew for regulating its secondary metabolism system and increasing the content of taxol.

Description

technical field [0001] The invention relates to the application field of plant growth regulators, in particular to a method for increasing the content of taxus taxol by using plant growth regulators. Background technique [0002] Paclitaxel is a diterpenoid alkaloid with complex structure in the bark of Taxus, which widely exists in the Taxus genus. It is a new generation of cancer chemotherapy drugs with unique physiological activities. Commercial paclitaxel is mainly derived from the bark of Taxus chinensis, which is an extremely slow-growing tree species, and the content of paclitaxel in the bark is also very low, accounting for only 0.01% of the dry weight. The amount of paclitaxel required to treat a patient is equivalent At 60 pounds of yew bark, that means 4 large trees will be felled. Although the yew plant is widely distributed, due to the destruction of some regional resources, the natural forest trees are decreasing day by day, and it has become an endangered and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P21/00
Inventor 董学会李召虎段留生张明才何钟佩
Owner CHINA AGRI UNIV
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