Method of utilizing three-dimensional rack seed cell double phase inoculation to construct engineered tissue

A three-dimensional scaffold and seed cell technology, applied in the field of biomedical engineering, can solve the problems of long time, complicated operation, and inability to generate, and achieve the effects of short time, good controllability and easy operation.

Inactive Publication Date: 2003-01-22
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Abstract
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  • Application Information

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Problems solved by technology

The above-mentioned prior art has the following defects: complex operation, special bioreactor is needed, the recombination rate of cells and scaffold material is low, cells still grow two-dimensionally in the scaffold, and it takes a long time (8 -12 weeks)
It still requires a dedicated bioreactor, the recombination rate of cells and scaffold materials is low, cells still grow two-dimensionally in the scaffold, and in vitro culture cannot produce a three-dimensional structure consistent with the scaffold material.

Method used

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  • Method of utilizing three-dimensional rack seed cell double phase inoculation to construct engineered tissue
  • Method of utilizing three-dimensional rack seed cell double phase inoculation to construct engineered tissue
  • Method of utilizing three-dimensional rack seed cell double phase inoculation to construct engineered tissue

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Embodiment Construction

[0023] The method of the present invention is applied to the in vitro construction of cartilage engineered tissue to illustrate the specific implementation of the present invention.

[0024] 1. Preparation of biogel solution:

[0025] Under aseptic conditions, the tendon fiber tissue of rat tail was cut into 1-2 mm, soaked in glacial acetic acid with a volume ratio of 0.001 for 72 hours, and fully shaken once every 8 hours. Centrifuge at a speed of 10,000-15,000 rpm, take the supernatant, add 0.1N sodium hydroxide to adjust the pH value to 6.5-7.5, and then centrifuge at a speed of 10,000-15,000 rpm, discard the supernatant Add an appropriate amount of glacial acetic acid with a volume ratio of 0.001 to dissolve the precipitate to make a preliminary purified liquid biogel. Take a small amount of sample to measure the protein content.

[0026] 2. Sterile hydration of tissue engineering three-dimensional scaffold materials

[0027] Using calcium polyphosphate fiber (Calium pol...

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Abstract

The present invention belongs to biomedicine engineering and is one method of constructing engineered tissue. The present invention features that biogel liquid is first prepared and them mixed with seed cell to form cell-biogel suspension, which exists first in liquid state and then in solid in certain condition, to realize double phase inoculation of seed cell in 3D rack. The tree parts of seed cell, biogel and 3D rack forms one united integral and this can realize the efficient combination between cell and material without loss or dropping, maintain the 3D homogeneous distribution of cell in material and maintain the initial form of the engineered tissue. The method of the present invention has simple operation, easy control and short culture period.

Description

1. Technical field [0001] The invention belongs to biomedical engineering, in particular to a method for constructing engineered tissue. 2. Background technology [0002] Tissue engineering research on human cartilage, bone, cornea, skin, muscle, liver and other tissues and organs is a subject of great concern in biomedical engineering today. The use of biomaterial technology to construct engineered tissues is the core content of tissue engineering research. In the research on the construction of engineered tissues, it was found that due to the insufficient compatibility of the three-dimensional porous scaffold material with the seed cells, the material only provided a limited surface area for the adhesion of the seed cells, and there were loss and a large number of shedding of the seed cells after inoculation. In addition, cells still grow two-dimensionally in the scaffold material, which is not conducive to the recovery or maintenance of their phenotype and the synthesis a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/00C12N5/07
Inventor 周强
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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