Scrophularia oligose, production method and use thereof

A production method and oligosaccharide technology, which are applied in the field of Scrophulariaceae oligosaccharides to achieve the effects of high yield, anti-tumor immune activity, and enhanced immune activity

Inactive Publication Date: 2003-03-12
SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI +1
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Literature reports, [Zhang Wenjie et al., Yunnan Botanical Research, 16(4), 407(1994)], [Li Yiming, Jiang Shanhao, Gao Wenyun, Zhu Dayuan, Acta Pharmaceutica Sinica, 34(6), 448(1999)], [Zou Chenting, Yang Xiuwei, Chinese Herbal Medicine, 3l(4), 241

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  • Scrophularia oligose, production method and use thereof
  • Scrophularia oligose, production method and use thereof
  • Scrophularia oligose, production method and use thereof

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[0026] Example 1

[0027] Wash and dry 500g of the crude drug Scrophulariaceae, pulverize, soak in 5 liters of deionized water at room temperature for 24 hours, and filter. The residue was continuously immersed in 2.5 liters of deionized water for 12 hours, filtered, and the filtrate was combined and concentrated under reduced pressure at 60°C to 1-2 liters. Then it was precipitated with 95% acetone, the final concentration of acetone was 80%, and the precipitation was carried out for 24 hours. Centrifuge, dissolve the precipitate with 1.25 liters of water, centrifuge, and dialyze in ion-free water for 48 to 72 hours. It was concentrated under reduced pressure at 60°C and freeze-dried to obtain 30g of total sugar of Scrophulariaceae. Add 2 liters of water to dissolve, add activated carbon treatment, repeat desorption twice, then concentrate under reduced pressure, centrifuge and freeze-dry to obtain 15 g of total sugar of Scrophulariaceae with a purity of 80%....

Example Embodiment

[0028] Example 2

[0029] Wash 500g of the crude drug Scrophulariaceae, dry, crush, soak in 5 liters of deionized water at room temperature for 36 hours, and filter. The residue was soaked in 2.5 liters of deionized water for 12 hours, filtered, and the filtrate was combined and concentrated under reduced pressure at 50°C to 2 liters. It was then precipitated with 95% acetone, the final concentration of acetone was 82%, and the precipitation was carried out for 48 hours. After centrifugation, the precipitate was dissolved in 2 liters of water, centrifuged, and dialyzed against ion-free water for 72 hours. After concentration under reduced pressure at 50°C and freeze-drying, 35 g of total sugar of Scrophularia cepacia is obtained. Add 2 liters of water to dissolve, add activated carbon treatment, repeat the removal three times, then concentrate under reduced pressure, centrifuge and freeze-dry to obtain 17 g of total sugar of Scrophulariaceae with a purity of 8...

Example Embodiment

[0030] Example 3

[0031]Scrophulariaceae total sugar is purified by DEAE-Cellulose column chromatography with a purity of 85-90%, and then desalted by Sephadex G-50 column chromatography and Sephadex G-10 to obtain Scrophulariaceae oligosaccharide SnS-2 with a purity of 98%. . The specific separation method: weigh 100 mg of the total sugar of Scrophulariaceae, dissolve it in 2-3 mL of distilled water, and centrifuge (4500r / min, 10min). Take the supernatant on a glass column (1.5cm×60cm) and eluted with 0.1M NaCl at a flow rate of 0.5mL / min. Collect 3-5mL in each tube, and about 11-17 tubes are absorption peaks. After collection, it was desalted by Sephadex G-10 and freeze-dried to obtain 60 mg of pure oligosaccharide SnS-2.

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Abstract

A process for extracting oligomeric saccharide SnS-2 from scrophularia root includes soaking in water, precipitating in organic solvent, dissolving precipitate in water, dialyzing supernatant, membrane separation, concentrating and drying. Its advantages are sure medical action for suppressing cancer and improving immunity, high yield and no toxic by-effect.

Description

technical field [0001] The invention relates to a homologous mixture of oligosaccharides extracted from Chinese medicinal materials, in particular to crocus oligosaccharides extracted from crude drug Scrophulariaceae Scrophulariae, its production method and application. technical background [0002] Scrophulariaceae is a traditional Chinese medicinal material, which has the effects of "cooling blood and nourishing yin, reducing fire, promoting body fluid, and detoxifying". Literature reports, [Zhang Wenjie et al., Yunnan Botanical Research, 16(4), 407(1994)], [Li Yiming, Jiang Shanhao, Gao Wenyun, Zhu Dayuan, Acta Pharmaceutica Sinica, 34(6), 448(1999)], [Zou Chenting, Yang Xiuwei, Chinese Herbal Medicine, 3l(4), 241(2000)], its chemical components mainly include alkaloids, flavonoid glycosides, sterols, amino acids, fatty acids, volatile oils, carotene, iridoid glycosides and trace elements, etc. Studies on sugars in Scrophulariaceae have not been reported yet. Contents ...

Claims

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Application Information

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IPC IPC(8): A61K31/702A61K31/715A61P35/00A61P37/04C07H3/06C08B37/00
Inventor 田庚元邓军娥
Owner SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI
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