Reformed lyophylization preparation of recombinant staphylokinase (r-Sak), its preparing method and application

Abstract

A freeze-dried preparation of recombinant glucokinase in the form free dried powder, injection, lipoplasm, or microcapsule for thrombolytic purpose contains the glucokinase with mutation of amino acids at positions 7, 3 and 43, and one or more of mannitol, phosphate, EDTA and sodium chloride. Its preparing process is also disclosed.

Description

technical field

[0001] The invention relates to a recombinant staphylokinase freeze-dried preparation, its preparation method and application as a thrombolytic drug, belonging to the technical field of biopharmaceuticals. Background technique

[0002] Cardiovascular and cerebrovascular diseases are currently the number one killer that endangers people's health, and thrombotic diseases such as acute myocardial infarction are the main causes of death of patients. Although there are some commercially available thrombolytic drugs in clinical practice, there are still many problems to be solved urgently in terms of their thrombolytic effect, specificity of thrombolysis and drug price. Staphylokinase (Staphylokinase, referred to as SAK) is a protein produced by Staphylococcus aureus with a molecular weight of 15.5KD. It can specifically bind to plasminogen, form a complex, and activate plasminogen to become Plasmin specifically degrades fibrin and dissolves thrombus. According t...