Microdrop oscillatory silicone groove polymeraxe chair reaction biological chip

A biochip and chain reaction technology, applied in the field of medical instruments, can solve the problems of difficult, low-cost mass production, difficult lab-on-a-chip, and long time.

A biochip and chain reaction technology, applied in the field of medical instruments, can solve the problems of difficult, low-cost mass production, difficult lab-on-a-chip, and long time.

CN1477208AInactive Publication Date: 2004-02-25TSINGHUA UNIV +1
2 Cites 12 Cited by

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microdrop oscillatory silicone groove polymeraxe chair reaction biological chip
  • Microdrop oscillatory silicone groove polymeraxe chair reaction biological chip
  • Microdrop oscillatory silicone groove polymeraxe chair reaction biological chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] A kind of droplet oscillation type silicon tank type polymerase chain reaction (PCR) biological chip embodiment of the present invention design, in conjunction with accompanying drawing, describe in detail as follows:

[0015] The structure of this embodiment is as image 3 , shown in 4, 5, 6, mainly by etching on the silicon substrate 21, and by the microchannel 24 (the droplet 22 of sample and reactant moves in it) that is closed by glass cover 23, three constant temperature working areas 16, inlet hole 15 and outlet hole 17; three constant temperature zones are separated by heat insulation tank 20, and two heaters 18 and one temperature sensor 19 are arranged symmetrically on each constant temperature zone. The thickness of the silicon base 21 is 300 μm; the microchannel 24 is 32,000 μm long, with a trapezoidal cross-section (formed according to the crystal orientation in bulk silicon processing), with an upper bottom width of 600 μm, a lower bottom width of 1000 μm...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
Login to View More

Abstract

The present invention relates to a microdrop shaking silicon groove type PCR biological chip, including microgroove and thermostatic region positioned in the back surface of microgroove and separatedby heat-insulating groove, resistive microtemperature sensor positioned on the back surface of the microgroove and microheaters which are symmetrically placed at two sides of said temperature sensor.The biological chip has extensive application in the fields of biological genome research, forensic medicine, genetic analysis, therapeutic effect evaluation and medical diagnosis, etc.

Description

technical field [0001] The invention belongs to the field of medical instruments, in particular to the design of a nucleic acid PCR clinical diagnosis biochip suitable for high throughput, rapidity and automation. Background technique [0002] Polymerase chain reaction (PCR, Polymerase Chain Reaction) is one of the important means of modern nucleic acid analysis. It can amplify the number of target nucleic acid molecules nearly doubled in one cycle, and after 20-30 cycles, the limited nucleic acid The molecule was amplified 109 times, thereby achieving the enrichment of the target molecule and preparing for further analysis. The birth of the PCR process has greatly accelerated the process of research on the genome structure of various organisms, and has a wide range of applications in forensic science, genetic analysis, treatment effect evaluation, and medical diagnosis. [0003] In recent years, with the development of micro-processing technology, microchips used in clinic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
25 Feb 2004
Publication
CN1477208A
IPC
C12P19/34; C12Q1/68
Inventors
李志信; 罗蓉