Tissue cultivation quick breeding method for Dysosma versipellis

Abstract

The present invention discloses the tissue cultivation process for quickly breeding Dysosma versipellis. The process includes selection and processing of explant, inducing culture, proliferating culture, rooting culture, transplanting and other steps. The present invention uses MS culture medium with added auxiliary agents including active carbon, 6-benzyl adenine, gibberellin and corn extract for two key stages including the inducing growth of fascicular seedling and rooting of robust seedling. The present invention solves the technological problems in fast breeding and stable rooting of Dysosma versipellis, and has the advantages of high breeding coefficient, stable technology, short seedling growth period and low cost.

Description

Technical field

[0001] The invention relates to the field of biotechnology, in particular to a tissue culture and rapid propagation method of octagonal lotus. Background technique

[0002] Plants are a natural treasure house of medicines. People have a long history of using medicinal plants. About 75% of the world's population use plants as a source of medicines for the treatment and prevention of diseases (Xing Jianmin, 2001). Mankind has discovered many drugs with high physiological activity from plants. Currently, drugs derived from plants account for more than 25% of the total drugs (Zheng Guangzhi, 1987). Traditional Chinese herbal medicine in my country has a history of thousands of years and is still widely used in my country and many countries and regions. However, traditional Chinese herbal medicines are obtained at the cost of collecting and consuming a large amount of wild plant resources. When the amount of collection and consumption exceeds the regeneration capacity ...