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Tissue cultivation quick breeding method for Dysosma versipellis

A tissue culture and rapid technology, applied in the biological field, can solve the problems of rapid propagation of star anise lotus tissue culture, poor reproduction mechanism, harsh habitat requirements, etc., to achieve the effect of eliminating the invasion of diseases and insect pests and pesticide residues, saving manpower, and uniform quality

Inactive Publication Date: 2005-03-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the harsh habitat requirements and poor reproductive mechanism of wild star anise, it is not only the reason for its endangerment, but also makes it very difficult for conventional tissue culture techniques to rapidly propagate it.
So far, there have been no reports on the success of tissue culture and rapid propagation of star anise lotus at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The buds of octagonal lotus collected from Mount Emei are washed with tap water, and then rinsed in running water for half an hour, then the surface is disinfected. The disinfection method is: soak in 70% alcohol for 30s, rinse twice with sterile water, and then use 0.1% liters 100ml mercury (add 2-3 drops of Tween T-20) to sterilize for 8 minutes, and finally rinse with sterile water. Connect the sterilized medium MS+BA 0.5mg / l+ZT1.0mg / l. After culturing for 15 days at 25±2℃, it can be seen that there are about 5 new buds appearing on each explant. Take out the explants with buds under aseptic conditions, separate each bud with a scalpel, and place them in a culture vessel containing the same medium as above. After 20 days, the buds in each culture vessel can be Several new shoots are regenerated. Then separate each bud and transfer it to MS+BA1.0mg / l+ZT0.5mg / l+GA0.2mg / l+PVP0.5mg / l for continuous cultivation to achieve the purpose of rapid growth. The oxidant PVP prevents ...

Embodiment 2

[0041] The buds of octagonal lotus collected from Mount Emei, washed with tap water, rinsed in running water for half an hour, soaked in 70% alcohol for 30s, rinsed twice with sterile water, and then 100ml of 0.1% mercury (add 2-3 drops Tween T-20) sterilize for 15 minutes, and finally rinse with sterile water. Blot dry with sterile paper, cut into about 1 cm, and inoculate in medium MS+BA 0.5mg / l+NAA 0.2mg / l+GA 0.2mg / l. The culture temperature is 25±2℃, the light intensity is 1500LX, and the light time is 10-12h / d. After culturing for 20 days, 5-7 new shoots appeared on the explants. Take out the explants with several buds under aseptic conditions, cut each bud with a scalpel, and place them in 1 / 2 MS+BA 0.2mg / l+IBA0.5mg / L+PVP0. Rooting culture on 05% medium. It takes about 30 days to take root. The rooted plants are taken out, placed under natural temperature and light conditions for 5-7 days, the bottle cap is opened, the medium is washed, and the seedlings are transplanted on ...

Embodiment 3

[0043] The buds of octagonal lotus collected from Mount Emei are washed with tap water, and then rinsed in running water for half an hour, then the surface is disinfected. The disinfection method is: soak in 70% alcohol for 30s, rinse twice with sterile water, and then use 0.1% liters 100ml mercury (add 2-3 drops of Tween T-20) sterilize for 10 minutes, and finally rinse with sterile water. Connect the sterilized medium MS+BA 0.5mg / l+ZT1.0mg / l. Cultivate for 1 to 2 days under dark conditions, and then transfer to 40W double-tube fluorescent lamp for cultivation, the light intensity is 2000lx, the temperature is kept at 25±2℃, and the culture is 15 days. It can be seen that there are about 5 new buds appearing on each explant. Take out the explants with buds under aseptic conditions, separate each bud with a scalpel, and place them in a culture vessel containing the above continuous culture medium. Within 10-20 days, The bud can regenerate several new buds. The explants grown on th...

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PUM

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Abstract

The present invention discloses the tissue cultivation process for quickly breeding Dysosma versipellis. The process includes selection and processing of explant, inducing culture, proliferating culture, rooting culture, transplanting and other steps. The present invention uses MS culture medium with added auxiliary agents including active carbon, 6-benzyl adenine, gibberellin and corn extract for two key stages including the inducing growth of fascicular seedling and rooting of robust seedling. The present invention solves the technological problems in fast breeding and stable rooting of Dysosma versipellis, and has the advantages of high breeding coefficient, stable technology, short seedling growth period and low cost.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to a tissue culture and rapid propagation method of octagonal lotus. Background technique [0002] Plants are a natural treasure house of medicines. People have a long history of using medicinal plants. About 75% of the world's population use plants as a source of medicines for the treatment and prevention of diseases (Xing Jianmin, 2001). Mankind has discovered many drugs with high physiological activity from plants. Currently, drugs derived from plants account for more than 25% of the total drugs (Zheng Guangzhi, 1987). Traditional Chinese herbal medicine in my country has a history of thousands of years and is still widely used in my country and many countries and regions. However, traditional Chinese herbal medicines are obtained at the cost of collecting and consuming a large amount of wild plant resources. When the amount of collection and consumption exceeds the regeneration capacity ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01H3/02A01H3/04A01H4/00
Inventor 傅承新潘琦姜维梅陈士超
Owner ZHEJIANG UNIV
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