Preparation method of detection filter membrane used for bacteria and virus sampling in air

A technology of bacteria and viruses, which is used in the field of preparation of filter membranes for sampling and detection of bacteria and viruses in the air, and can solve the problems of inability to interact with microorganisms and beneficial combination of retention efficiency

Inactive Publication Date: 2005-03-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] 2. Can not make a beneficial combination of microbial action and retention efficiency
From the point of view of th...

Method used

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  • Preparation method of detection filter membrane used for bacteria and virus sampling in air
  • Preparation method of detection filter membrane used for bacteria and virus sampling in air
  • Preparation method of detection filter membrane used for bacteria and virus sampling in air

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Place the supporting porous membrane on a horizontal glass plate, and coat the ready-to-use membrane-making solution (0.75% chitosan solution) on the surface of the supporting porous membrane. Adjust the height of the membrane-making knife to move at a speed of 5 cm per minute Knife holder, so that the membrane-forming solution is evenly distributed on the porous membrane, and then heat-treated at 40°C for 3 hours.

Embodiment 2

[0027] Place the supporting porous membrane on a horizontal glass plate, and coat the ready-to-use membrane-making solution (0.75% chitosan solution) on the surface of the supporting porous membrane. Adjust the height of the membrane-making knife to move at a speed of 5 cm per minute Knife holder, so that the membrane-forming solution is evenly distributed on the supporting porous membrane, and then heat-treated at 80°C for 1 hour.

Embodiment 3

[0029] Place the supporting porous membrane on a horizontal glass plate, and coat the ready-to-use membrane-making solution (0.75% chitosan solution) on the surface of the supporting porous membrane. Adjust the height of the membrane-making knife to move at a speed of 6 cm per minute Knife holder, so that the membrane-forming solution is evenly distributed on the porous membrane, and then heat-treated at 40°C for 3 hours.

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Abstract

The invention relates to a kind of manufacturing processes of composite membrane for microbes sampling detection in air. Put bracing porous membrane on horizontal glass plate, regulate the high of bar for manufacturing membrane, and then move knife rest by 5-6 cm per minute, in order to make manufacturing membrane liquor distribute evenly in the surface of porous membrane and form the surface layer of the composite membrane. After the reaction temperature is 20-30 centigrade, the reaction humidity is 20-50%, and the pressure is the negative pressure that lowers than atmospheric, heat-treat it with the temperature of 40-80 centigrade by 1-3 hours, and at the same time it can make the composite membrane of different aperture by regulating the high of bar for manufacturing membrane. The macromolecule substance of the composite membrane can hold activity of the microbe held back in certain extent. Compared with others filtration film, the composite membrane can decline the formation of filter cake, and in the same testing condition, its flux is obviously higher and higher than others filtration in the same aperture. The using coating solution of the macromolecule has biological activity, so it can profit for holding activity of the microbe in a certain degree, can make the sample reflect the air status more truthfully, and can improve the holding problem of microbe activity in sampling in order to decline the error.

Description

technical field [0001] The invention relates to a preparation method of a filter membrane used for sampling and detecting bacteria and viruses in the air. Background technique [0002] The use of membrane filtration to collect aerosol particles is the most common method for aerosol sampling. The so-called aerosol refers to a dispersion system in which solid or liquid particles are suspended in a gaseous medium. Pasteur in 1861 and Petri in 1888 began to collect microbial aerosols through filter membranes. It is a simple and inexpensive method of collecting aerosol samples. [0003] In recent years, membrane filtration has been widely used for aerosol sampling. Many materials are used for filter membrane materials, the most important filter membrane materials are fibrous filter materials and porous membrane filter materials. [0004] Most of the fiber filter materials are arranged in the direction perpendicular to the air flow, the porosity is 70-...

Claims

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Application Information

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IPC IPC(8): B01D69/10B01D71/08
Inventor 陈延京张林陈欢林程丽华宋明宇谢林盛吉芳
Owner ZHEJIANG UNIV
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