Method for preparing composite micro-ecological enzyme for feeding the ruminant by using disused mushroom dregs

A technology of compound microecology and ruminants, which is applied in the production of ruminants and in the field of compound microecological enzyme preparations for ruminant feeding, can solve the problems of low comprehensive enzyme activity, low comprehensive enzyme activity, and unstable enzyme activity, etc., and achieve Huge economic benefits, reduced phosphorus content, and the effect of reducing disease incidence

Inactive Publication Date: 2005-08-17
迟乃玉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The annual production of single-enzyme micro-ecological feed enzyme preparations in my country is less than 1000T (mainly used for monogastric animals), and the comprehensive enzyme activity is low, the enzyme activity is unstable, and the price is high (100,000 to 120,000 yuan / ton)
If the price of compound microecological feed enzyme preparation is 40,000 to 60,000 yuan / ton, my country will need hundreds of thousands of tons per year. At present, the strains officially approved for the production of enzyme preparations for monogastric animals mainly include Bacillus cereus and Bacillus subtilis , Lactobacillus, Lactococcus, etc. Most of the strains produced are single strains, and most of them are used as therapeutic agents for preventing and treating diarrhea in livestock and poultry. Only a few feed companies are in trial production, and the comprehensive enzyme activity is not high
However, the microecological enzyme preparation in the field of ruminant breeding has not yet been applied and developed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1), protease-producing bacteria, lipase-producing bacteria, cellulase-producing bacteria, amylase-producing bacteria and lactic acid bacteria were activated according to the strain instructions provided by CGMCC, and gradually expanded and cultivated to prepare bacterial agents. Activity unit (SI unit, cellulase activity unit is based on FPA activity unit) as the unit of measurement, according to protease: lipase: amylase: cellulase = (0.4 ~ 0.7): (0.2 ~ 0.4): (0.4 ~0.8): (0.8~1.5) ratio, prepare compound microorganism liquid inoculum; On the basis of the compound microorganism liquid inoculum of above-mentioned preparation, add the lactic acid bacteria inoculum of 3%~12% (volume ratio) again, promptly prepare It is an enzyme-producing composite microbial bacterial agent used in the fermentation production of the compound microecological enzyme preparation for feeding ruminants of the present invention.

[0014] (2) Mushroom dregs (non-polluting) produced after harvest...

Embodiment 2

[0020](1), protease-producing bacteria, lipase-producing bacteria, cellulase-producing bacteria, amylase-producing bacteria and lactic acid bacteria were activated according to the strain instructions provided by CGMCC, and gradually expanded and cultivated to prepare bacterial agents. Activity unit (SI unit, cellulase activity unit is based on FPA activity unit) as the unit of measurement, according to protease: lipase: amylase: cellulase = (0.4 ~ 0.7): (0.2 ~ 0.4): (0.4 ~0.8): (0.8~1.5) ratio, prepare compound microorganism liquid inoculum; On the basis of the compound microorganism liquid inoculum of above-mentioned preparation, add the lactic acid bacteria inoculum of 3%~12% (volume ratio) again, promptly prepare It is an enzyme-producing composite microbial bacterial agent used in the fermentation production of the compound microecological enzyme preparation for feeding ruminants of the present invention.

[0021] (2) Mushroom dregs (non-polluting) produced after harvesti...

Embodiment 3

[0027] (1), protease-producing bacteria, lipase-producing bacteria, cellulase-producing bacteria, amylase-producing bacteria and lactic acid bacteria were activated according to the strain instructions provided by CGMCC, and gradually expanded and cultivated to prepare bacterial agents. Activity unit (SI unit, cellulase activity unit is based on FPA activity unit) as the unit of measurement, according to protease: lipase: amylase: cellulase = (0.4 ~ 0.7): (0.2 ~ 0.4): (0.4 ~0.8): (0.8~1.5) ratio, prepare compound microorganism liquid inoculum; On the basis of the compound microorganism liquid inoculum of above-mentioned preparation, add the lactic acid bacteria inoculum of 3%~12% (volume ratio) again, promptly prepare It is an enzyme-producing composite microbial bacterial agent used in the fermentation production of the compound microecological enzyme preparation for feeding ruminants of the present invention.

[0028] (2) Mushroom dregs (non-polluting) produced after harvest...

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PUM

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Abstract

Waste mushroom growing medium after picking up mushroom is compounded with crushed crop stalk, and the mixture is moistened with water, inoculated with 2-8 % enzyme producing composite microbial preparation and fermented at certain temperature for 3-15 days to convert all the material into semi-solid as composite micro ecological enzyme preparation for ruminant. After finishing the fermentation, the product is detected to possess total microbe amount not less than 3.0E10 CFU/g, cellulase FPA not less than 400 U/g, proteinase not less than 180 U/g, lipase not less than 240 U/g and amylase not less than 200 U/g.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a compound for feeding ruminants with micro-ecological effects, which uses waste mushroom dregs produced after the production of edible fungi as the main raw material and is artificially domesticated through the action of a variety of beneficial enzyme-producing microorganisms. A production method of a microecological enzyme preparation, which is applied to the production of ruminants. Background technique [0002] With the entry of WTO, the value of conventional agricultural products has depreciated, resulting in increased production but not income for farmers. The animal husbandry production has shown broad development prospects, especially the economic benefits of raising ruminants such as cattle and sheep are more prominent. Although the business opportunities and potential of the cattle and sheep breeding industry are huge, with the implementation of my country's "...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/30A23K20/189A23K50/10
Inventor 迟乃玉张庆芳
Owner 迟乃玉
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