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Triazoline artificial semi-antigen, antigen, specific antibody and use thereof

A technology of artificial haptens and artificial antigens, which is applied in the field of antigens and specific antibodies, can solve the problems of cumbersome, complicated and poisonous processes, and achieve the effect of low cross-reaction rate and good affinity

Inactive Publication Date: 2005-10-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Triazophos original drug is moderately toxic and toxic to fish and bees
However, this method requires complex instruments that most laboratories do not have, and the process is cumbersome, so it is not suitable for screening and testing of large batches of samples

Method used

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  • Triazoline artificial semi-antigen, antigen, specific antibody and use thereof
  • Triazoline artificial semi-antigen, antigen, specific antibody and use thereof
  • Triazoline artificial semi-antigen, antigen, specific antibody and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of triazophos artificial hapten, molecular structural formula is (n=3 at this moment):

[0035]

[0036] It is used as the raw material of the antigen system for animal immunization.

[0037] A kind of triazophos artificial antigen made by above-mentioned triazophos artificial hapten, molecular structural formula is:

[0038]

[0039] A triazophos-specific antibody is monoclonal or polyclonal immunoglobulin G obtained by immunizing rabbits or white mice with the above-mentioned triazophos artificial antigen and capable of specific immune reaction with triazophos. It is used to detect the residues of triazophos in food, agricultural products and environmental samples; the environmental samples are soil samples or water samples.

[0040] The above-mentioned triazophos artificial hapten, antigen and antibody preparation method are as follows:

[0041] 1. Synthesis of O-ethylthiophosphoryl dichloride (TZLM-1)

[0042] Weigh the phosphorus trichloride (PSCl ...

Embodiment 2

[0073] A kind of triazophos artificial hapten, molecular structural formula is (n=5 at this moment):

[0074]

[0075] A kind of triazophos artificial antigen made by above-mentioned triazophos artificial hapten, molecular structural formula is:

[0076]

[0077] A triazophos-specific antibody is monoclonal or polyclonal immunoglobulin G obtained by immunizing rabbits or white mice with the above-mentioned triazophos artificial antigen and capable of specific immune reaction with triazophos. It is used to detect the residues of triazophos in food, agricultural products and environmental samples; the environmental samples are soil samples or water samples.

[0078] The above-mentioned triazophos artificial hapten, antigen and antibody preparation method are as follows:

[0079] 1. Synthesis of O-ethylthiophosphoryl dichloride (TZLM-1)

[0080] Weigh the phosphorus trichloride (PSCl 3 ) 68g (about 0.4mol) was placed in a three-necked flask with a low-temperature thermom...

Embodiment 3

[0100] A kind of triazophos artificial hapten, molecular structure formula is (n=1 at this moment)

[0101]

[0102] It is used as the raw material of the antigen system for animal immunization.

[0103] A kind of triazophos artificial antigen made by above-mentioned triazophos artificial hapten, molecular structural formula is:

[0104]

[0105] A triazophos-specific antibody is monoclonal or polyclonal immunoglobulin G obtained by immunizing rabbits or white mice with the above-mentioned triazophos artificial antigen and capable of specific immune reaction with triazophos. It is used to detect the residues of triazophos in food, agricultural products and environmental samples; the environmental samples are soil samples or water samples.

[0106] The above-mentioned triazophos artificial hapten, antigen and antibody preparation method are as follows:

[0107] 1. Synthesis of O-ethylthiophosphoryl dichloride (TZLM-1)

[0108] Weigh the phosphorus trichloride (PSCl 3 ) ...

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Abstract

Triazophosphous artificial semi-antigen, antigen, specific antibody and its use are disclosed. N=1-10 in molecular structural formula. It achieves high titer, specificity and affinity.

Description

technical field [0001] The present invention relates to selecting a compound having -COOH and possibly containing the original structure of triazophos as the triazophos hapten, antigens and specific antibodies made from this hapten; and such haptens and antigens , the use of specific antibodies. Background technique [0002] The invention belongs to the technical field of immunochemistry and residue analysis of small-molecule pesticide compounds (molecular weight less than 1000 Daltons), relates to organic synthesis, immunochemistry and biochemistry, and relies on basic principles of immunology and immunochemistry and biotechnological means to design and synthesize Small molecule target analyte hapten, and coupled with carrier protein to prepare effective artificial antigen, immunize animals to prepare specific antibody for small molecule analyte, use the specific immunological reaction of antigen and antibody and the identification of markers that are easily detected and id...

Claims

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Application Information

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IPC IPC(8): C07F9/6518C07K1/107C07K16/18C07K17/02G01N33/563
Inventor 桂文君程敬丽朱国念
Owner ZHEJIANG UNIV
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