Gas-phase molecular ion reactor based on radio-frequency quard-polar rod, its realization and use

An ion reactor and radio frequency quadrupole technology, applied in the field of tandem mass spectrometry, can solve problems such as the difficulty of spectrum resolution, and achieve the effect of good controllability and high sensitivity

An ion reactor and radio frequency quadrupole technology, applied in the field of tandem mass spectrometry, can solve problems such as the difficulty of spectrum resolution, and achieve the effect of good controllability and high sensitivity

CN1779457AActive Publication Date: 2006-05-31KUSN HEXIN MASS PECTRUM TECH +1
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  • Gas-phase molecular ion reactor based on radio-frequency quard-polar rod, its realization and use
  • Gas-phase molecular ion reactor based on radio-frequency quard-polar rod, its realization and use
  • Gas-phase molecular ion reactor based on radio-frequency quard-polar rod, its realization and use

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] figure 1 shows the structure of the present invention, by figure 1 It can be seen that the gas-phase molecular ion reactor device based on the radio frequency quadrupole is connected to the mass spectrometer 8 through the outlet 7, and is placed together in a differential vacuum system 9; the device includes a radio frequency quadrupole, and the radio frequency quadrupole Set in the reaction chamber 2, the vacuum degree in the reaction chamber can be adjusted by the ion inlet 1, and the reaction gas can be introduced from the gas guide port 3 through the capillary. Each rod of the RF quadrupole is composed of three sections, so that the RF quadrupole forms three stages, the first stage 4 is a mass filter, the second stage 5 is a molecular ion reactor, and the third stage 6 is an ion focusing transporter , there is a gap between the three-stage radio frequency quadrupoles; figure 2 Shown as a cross-sectional view of the present invention, an axial field pole piece 10 ...

Embodiment 2

[0041] The device of this embodiment is the same as that of Embodiment 1, and the treated polypeptide sample is also the same as that of the embodiment. This device and implementation method are mainly used for the analysis of impurity ions. Since I am not interested in this polypeptide ion, I want to analyze its impurities, and the polypeptide is The main component of the sample has an impact on the analysis of impurities, and it is hoped to remove it before entering the mass spectrometer; the specific operation is: use figure 1 The RF voltages applied to the three quadrupoles 4, 5, and 6 are the same, but the excitation voltage is not applied to the quadrupoles 5 and 6. At this time, the quadrupoles 5 and 6 only play the role of ion transmission focusing; while the first stage The quadrupole 4 is used as a mass filter, and the ion excitation frequency with a mass-to-charge ratio of 571 is selected, and the amplitude is relatively high, such as 5V, so that the excited ions hit...

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Abstract

A method for realizing gas phase molecule ¿C ion reaction based on radio frequency quadrupole rod includes imposing electric field on said quadrupole rod to let ion analyzed obtain kinetic energy and to let it generate molecule ¿C ion reaction with background gas molecule to generate subion of ion to be analyzed, using mass analyzer to measure subion for realizing cascade mass spectrum analysis of ion to be analyzed. A device of gas phase molecule ¿C ion reactor based on radio frequency quadrupole rod is also disclosed.

Description

technical field [0001] The invention relates to molecular ion reaction technology used in the field of tandem mass spectrometry, in particular to a gas phase molecular ion reactor device based on a radio frequency quadrupole and its realization method and application. Background technique [0002] In the field of mass spectrometry, in order to analyze the lower content components in mixed samples, it is necessary to remove the background ions of high concentration components, and ion filtration technology is needed at this time. At the same time, as the molecular weight of the sample to be analyzed increases, isomers are very common, and the structural information of the molecule is becoming more and more important, such as the amino acid sequence of proteins and polypeptides, the structure of drugs, and the metabolites of drugs in the human body. The adducting position in DNA, the adducting position of toxic pollutants in DNA fragments, DNA base seq...

Claims

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Application Information

Patent Timeline
31 May 2006
Publication
CN1779457A
IPC
G01N30/72; H01J49/04; H01J49/26
Inventors
周振; å‚…å¿