Method and system for quantitatively fast level testing flow

A flow system and horizontal technology, applied in the field of qualitative and quantitative rapid horizontal lateral flow, can solve the problems of ineffective blocking of cells, leakage of red blood cells, background that cannot meet the sensitivity/specificity requirements, etc., and achieve relative volume independence , reduce the difference between boxes, and ensure the accuracy of the effect

Inactive Publication Date: 2006-06-14
RELIA BIOTECH SHENZHEN LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] There have been many attempts to establish a rapid horizontal lateral flow method for whole blood detection (such as US Patent US 6,136,610; US Patent US 6,528,323, WO03 / 3008933, US Patent US 5,766,552, etc.), but all failed because the above conditions cannot be met at the same time
They either cannot effectively block cells, causing red blood cells to leak or hemolyze, causing the background (background) to be too high to meet the sensitivity / specificity requirements; or due to the low filtration efficiency of liquid samples, the volume is too small to complete the required Biochemical reactions are required; or the rapid detection cannot be completed in a short period of time because the filtration speed is too slow; or because the detection method itself requires strict volume dependence, it cannot overcome the difficulty of the difference in hematocrit volume in individual whole blood samples; or and How it works is not explored / elaborated, calling into question the reproducibility of its results and the scalable productivity of its structure

Method used

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  • Method and system for quantitatively fast level testing flow
  • Method and system for quantitatively fast level testing flow
  • Method and system for quantitatively fast level testing flow

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Screening and filtering structure of whole blood sample port 1 filter membrane (Attached Tables 1, 2, 3, 4).

[0067] material:

[0068] 1. Filtration membrane: Ahlstron Filtration (United States)

[0069] Grade 111: glass fiber

[0070] Grade 141 and Grade 142: glass fiber

[0071] Grade 1660, Grade 1661, Grade 1662 and Grade 1663

[0072] 2. Rabbit anti-human red blood cell antibody (China Ruitai Tesi)

[0073] 3. Filter membrane pretreated with anti-human red blood cell antibody (refer to procedure)

[0074] 4. HIV(-) whole blood and plasma samples

[0075] 5. HIV(+) whole blood and plasma samples

[0076] 6. HIV test kit

[0077] Attach Figure 5 Structure to assemble the test box for HIV antibody detection.

[0078] Compare:

[0079] 1. The effect of separating red blood cells after treating different filter membranes with anti-human red blood cell antibodies.

[0080] 2. The migration speed of the filtered plasma on the NC membrane.

[0081] 3. The difference of HIV(+...

Embodiment 2

[0094] The effect of adding or not adding double-sided tape at port 1 on the flow direction of the filtered sample

[0095] Purpose: 1. To observe the influence of different types of nitrocellulose membrane (NC membrane) with or without double-sided tape on the flow direction of plasma samples at port 1

[0096] 2. Observe the influence of different types of NC membrane port 1 with or without double-sided tape on the flow direction of the whole blood filtration sample

[0097] Material: 1, 5mm wide x 100mm long HF135NC film, American MILLIPORE

[0098] 2. 5mm wide x 100mm long HF90NC film American MILLIPORE

[0099] 3,5mm×5mm double-sided tape China

[0100] 4. 141 whole blood sample filter pad (5 mm wide x 7.5 mm long) treated with 0.2 mg / ml mouse anti-human red blood cell antibody

[0101] 5. 30cm long steel ruler China

[0102] 6. Whole blood and plasma samples from normal people, samples from clinical blood donors

[0103] Design: 50ul sample addition------timekeeping------mea...

Embodiment 3

[0111] The selection of the filter membrane and the filter structure of the whole blood sample port 2 when adding samples (Attached Tables 6A, 6B, 6C).

[0112] purpose:

[0113] Establish the best whole blood filtration structure suitable for sample application to port 2 so that the blood can be used for bidirectional horizontal lateral flow detection.

[0114] material:

[0115] 1. Whole blood separation membrane: Ahlstrom Filtering, USA

[0116] GRADE CYTOSEP Catalog Number 1661 Lot Number: 2050401

[0117] GRADE CYTOSEP Catalog Number 1660 Lot Number: 2050401

[0118] GRADE CYTOSEP Catalog Number 142 Lot Number: 2050401

[0119] GRADE CYTOSEP Catalog No. 141 Lot No.: 2050401

[0120] GRADE CYTOSEP Catalog Number 111 Lot Number: 2050401

[0121] 2. Chemical coupling pad (not coated with colloidal gold): Millipore China Co., Ltd.

[0122] 3. Nitrocellulose membrane: Millipore China Co., Ltd.

[0123] 4. Mouse anti-human erythrocyte antibody (Bosen, Xiamen, China).

[0124] 5. Fi...

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Abstract

The invention relates to quantitative fast horizontal side flow method and system. It includes the following steps: practicing each detecting item for liquid state biological specimen on biochemical reaction platform; using filtration to separate cell from liquid state biological specimen to reach the need liquid volume; the biochemical reaction platform is made up test strip and testing cassete shell; the biological specimen is added at any one of the application of sample port of the shell; forming effective capillary chromatography differential pressure to make the filtered liquid and the dissolved measured matter do orientation flow on the platform; judging detecting result according to optical density. Thus the invention can do quantitative fast detection for whole blood or other cell biology, and realize relative volume non dependence of detecting result to ensure its accuracy.

Description

Technical field [0001] The present invention relates to a qualitative and quantitative rapid horizontal lateral flow method and system, which is suitable for analyzing substances to be tested in biological samples (especially biological samples containing whole blood, or red blood cells, or white blood cells or other cells) to obtain Qualitative or quantitative analysis results. Background technique [0002] Quantitative, rapid and sensitive diagnostic methods are the development direction of the increasingly popular "Point of care test" (POCT). But the real "fast" requires that the specimens to be inspected are directly tested without any treatment. However, biological specimens often contain cells. For example, blood contains a large number of red blood cells, white blood cells, and other types of cells. Before performing the test, a time-consuming separation step is often required to remove the cells in the specimen to be tested. Moreover, due to the difference in hematocrit b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N1/28G01N33/50G01N33/543
Inventor 周思亮刘宁威廉·吉·罗特
Owner RELIA BIOTECH SHENZHEN LTD
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