Novel medicinal uses of icariin

Abstract

The invention provides the use of icraiin in preparing medicament for treating or preventing impairment caused by bacterial infection, more specifically for treating septicemia, endotoxemia and endotoxin shock. The invention also relates to the use of icraiin in preparing medicament for enhancing curative effects of antibiotics.

Description

technical field

[0001] The present invention relates to the application of icariin in the prevention or treatment of damage caused by bacterial infection and the application of drugs for enhancing the curative effect of antibiotics, in particular to the application of icariin in the prevention or treatment of sepsis, endotoxemia and endotoxin caused by bacterial infection. Application of drugs in shock and enhancing the efficacy of antibiotics. Background technique

[0002] Bacterial toxins are divided into endotoxins and exotoxins. Endotoxins are lipopolysaccharides in the cell wall of Gram-negative bacteria. They are located in the outermost layer of the cell wall. The biological activity is very complex and has a wide range of effects on the body. It can activate a variety of inflammatory cells to release inflammation. It can cause severe and complex pathological changes in organs, and can cause shock, disseminated vascular coagulation (DIC), adult respiratory distress sy...

Examples

preparation example 1

[0012] Preparation Example 1: Preparation of Icariin Injection

[0013] Weigh 1.00g of icariin, add 20ml of n-butanol and 30ml of propylene glycol to completely dissolve the saponin, add 0.15g of tetracaine hydrochloride and 0.02g of citric acid into 40ml of water for injection to dissolve completely, and mix the above aqueous solution Add to the drug solution and mix well; adjust the pH value to 5.5±1.0 with borax and boric acid; add 0.1% activated carbon for needles and keep warm at 85°C for 30 minutes, G 3 Filtrate through a vertically fused glass funnel, and filter through a 0.22um microporous membrane; the filtrate is divided into 7ml vials, each containing 2ml. Or lyophilized by a lyophilizer to make a lyophilized powder.

[0014] Icariin

[0015] Dissolve icariin, soy lecithin and cholesterol in ethanol and add them to refined soybean oil, mix until clear, and remove the alcohol by rotary evaporation or nitrogen evaporation to obtain icariin 10mg / ml and 50mg / ...

experiment example 1

[0016] Experimental example 1 Effect test of icariin on endotoxin lethality in mice

[0017] 1 material

[0018] Icariin injection: prepared according to Preparation Example 1.

[0019] Endotoxin: Department of Microbiology, Shanghai Second Military Medical University, batch number: 020412.

[0020] Second-class Kunming mice, weighing 19-21 g, were provided by the Experimental Animal Center of Shandong Provincial Natural Medicine Engineering Technology Research Center, certificate number: SYXK (Lu) 20030020.

[0021] Dexamethasone sodium phosphate injection: Tianjin Pharmaceutical Lianshui Co., Ltd., specification: 5mg, batch number: 030825.

[0022] 2 Methods and results

[0023] Mice were randomly divided into blank control group, dexamethasone group, icariin 400mg / kg group, 100mg / kg group, 50mg / kg group, 20mg / kg group, 10mg / kg group, 20 in each group, male and female Half. After intravenous injection of endotoxin 20mg / kg, the mice were administered intravenously, and t...

technical field

[0001] The present invention relates to the application of icariin in the prevention or treatment of damage caused by bacterial infection and the application of drugs for enhancing the curative effect of antibiotics, in particular to the application of icariin in the prevention or treatment of sepsis, endotoxemia and endotoxin caused by bacterial infection. Application of drugs in shock and enhancing the efficacy of antibiotics. Background technique

[0002] Bacterial toxins are divided into endotoxins and exotoxins. Endotoxins are lipopolysaccharides in the cell wall of Gram-negative bacteria. They are located in the outermost layer of the cell wall. The biological activity is very complex and has a wide range of effects on the body. It can activate a variety of inflammatory cells to release inflammation. It can cause severe and complex pathological changes in organs, and can cause shock, disseminated vascular coagulation (DIC), adult respiratory distress sy...

Examples

preparation example 1

[0012] Preparation Example 1: Preparation of Icariin Injection

[0013] Weigh 1.00g of icariin, add 20ml of n-butanol and 30ml of propylene glycol to completely dissolve the saponin, add 0.15g of tetracaine hydrochloride and 0.02g of citric acid into 40ml of water for injection to dissolve completely, and mix the above aqueous solution Add to the drug solution and mix well; adjust the pH value to 5.5±1.0 with borax and boric acid; add 0.1% activated carbon for needles and keep warm at 85°C for 30 minutes, G 3 Filtrate through a vertically fused glass funnel, and filter through a 0.22um microporous membrane; the filtrate is divided into 7ml vials, each containing 2ml. Or lyophilized by a lyophilizer to make a lyophilized powder.

[0014] Icariin

[0015] Dissolve icariin, soy lecithin and cholesterol in ethanol and add them to refined soybean oil, mix until clear, and remove the alcohol by rotary evaporation or nitrogen evaporation to obtain icariin 10mg / ml and 50mg / ...

experiment example 1

[0016] Experimental example 1 Effect test of icariin on endotoxin lethality in mice

[0017] 1 material

[0018] Icariin injection: prepared according to Preparation Example 1.

[0019] Endotoxin: Department of Microbiology, Shanghai Second Military Medical University, batch number: 020412.

[0020] Second-class Kunming mice, weighing 19-21 g, were provided by the Experimental Animal Center of Shandong Provincial Natural Medicine Engineering Technology Research Center, certificate number: SYXK (Lu) 20030020.

[0021] Dexamethasone sodium phosphate injection: Tianjin Pharmaceutical Lianshui Co., Ltd., specification: 5mg, batch number: 030825.

[0022] 2 Methods and results

[0023] Mice were randomly divided into blank control group, dexamethasone group, icariin 400mg / kg group, 100mg / kg group, 50mg / kg group, 20mg / kg group, 10mg / kg group, 20 in each group, male and female Half. After intravenous injection of endotoxin 20mg / kg, the mice were administered intravenously, and t...

Images