Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character

A hybrid wheat and blue-grain technology, which is applied in the field of two-line hybrid wheat breeding, can solve the problems of high risk, high cost of chemical andricides, and reduced pistil activity, so as to avoid the influence of bad genes and enhance heterosis , The effect of simple breeding method

Inactive Publication Date: 2006-09-06
NORTHWEST A & F UNIV
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AI Technical Summary

Problems solved by technology

[0003] Three-line CMS breeding is always 3-5 years behind conventional breeding, and it is difficult to surpass conventional breeding; twice outcrossing, high risk and high seed production cost (Sang Wei, Han Xinnian, etc., 2005; Liu Xunsheng, 2005)
However, there are also defects. For example, the effect of chemical killing is closely related to the dosage, processing time and the growth and development period of wheat. If the medication tool is not precise enough, improper medication will cause incomplete androcidal or reduced pistil activity, thereby affecting the purity and quality of hybrids. Seed Production
The cost of existing chemical andricides is relatively high, and the cost of using the agent for hybrids is about 0.70 yuan / kg, and the effect is not very satisfactory, which still affects the utilization of hybrid wheat

Method used

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  • Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character
  • Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character
  • Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character

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Experimental program
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Effect test

specific Embodiment approach

[0035] Establishment of ms-Rht3 gene linkage:

[0036] 116 single plants of 94.18.26 (genotype ms-rht3 / Ms-Rht3, ms sterility gene ms1b from the "Probus" mutant, Rht3 dwarf gene from Nainari Rht3) self-bred progeny were planted in the greenhouse, Since the Rht3 gene is not sensitive to gibberellic acid, it was treated with 10ppm gibberellic acid (GA3) at the seedling stage, and then the seedlings were classified according to height and transplanted to the field. Among the 29 high-stalk (80-95cm) populations, the rate of sterile plants was 93.1%, and among the 60 semi-dwarf (60-65cm) populations, the rate of sterile plants was 1.7%, that is, 2 plants were sterile and 27 plants were short. In the culm (40-45cm) population, the rate of sterile plants was 0%. Using these two medium-stalked sterile plants as female parents to cross with Australian wheat variety Cunningham, two genotypes will appear in the F1 generation, namely ms-Rht3 / Ms-rht3 (semi-dwarf) and ms-rht3 / Ms-rht3 ( tal...

Embodiment 1

[0043] 1) Embodiment 1 (method one):

[0044] The hybrid combination of the original dual-purpose line T0010-5B-1LB-1 and the elite line 949-14, the combination number is x02163, in F 1 For generations, only blue seeds are selected for planting, from F 2 The light blue seeds were sorted out from the generation seeds and planted, and the excellent individual plants were selected. Plant the white-grained and light-blue-grained seeds of each strain of the F3 generation separately, about 50 plants each, and select and harvest light-blue-grained strains with 25% or 100% sterile plants in the white-grained population and good agronomic properties . By analogy, until the strain is stable and the isolated white-grain populations are all sterile. X02163-8-1-8, X02163-8-1-12, X02163-8-7-1, X02163-8-7-2, X02163-8-7-4, X02163-8-7- 10 and some new light blue grain dual-purpose series. These dual-purpose lines are weak winter, strong tillering ability, medium resistance to stripe rust ...

Embodiment 2

[0045] 2) Embodiment 2 (method two):

[0046] Select the excellent sterile line isolated from the F2 generation of the cross between the sterile line X9701-1-1 and the excellent line JYP97-1-1 as the female parent to cross with the dual-purpose line T0010-5B-1LB-1, the combination number is X01098, and The first method is the same as F 1 Generation planting light blue seeds, F 2 Plant light blue seeds on behalf of others, and select excellent individual plants, F 3The excellent lines were screened above, but the difference was that only light blue seeds were planted in the remaining generations until the lines were stable and the white-seed male sterile line needed to be isolated. X01098-1-2-4-3-2, X01098-1-2-4-3-3, X01098-1-2-4-4-1, X01098-1-2-4-4- 2. X01098-1-2-4-4-11, X01098-1-2-4-4-13, X01098-1-2-4-4-15 and some new dual-purpose series of light blue grains. These dual-purpose lines are of weak spring sex, strong tillering ability, medium resistance to stripe rust and h...

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Abstract

The invention discloses a breeding method of bilinear wheat hybrid taking blue granule as mark property. The method firstly establishes original bilinear system, then establishes breeding method for new bilinear, and at last prepares hybrid combination to sift dominant combination for production. The invention provides a new way for using wheat hybrid advantage, reduces effectively the cost for hybrid breeding and producing and possesses wide application prospect. The breeding of sterile line will not be lagged behind from that of normal breed when producing hybrid by this invention; the fruit got from bilinear auto-copulation is normal and the breeding coefficient of sterile is large; the fruit of hybrid of sterile line with arbitrary good wheat strain is normal; the good cytoplasm of out source can be replanted into sterile line to improve hybrid advantage; there is no bad influence in the sterile gene and 4thS.4AgL additive displacement colorant layer.

Description

technical field [0001] The present invention relates to the fields of cytogenetics, chromosome engineering, biochemistry, wheat genetics and breeding, utilization of recessive sterility gene in wheat heterosis, etc., especially relates to a two-line method hybrid wheat with blue grain as a marker trait Breeding methods. Background technique [0002] Wheat is one of the main food crops in my country. With the reduction of arable land and the increase of population, it is imperative to increase the yield of wheat per unit area. The utilization of heterosis is an important way to increase wheat yield, and the hybridization of wheat varieties is the need and inevitable trend of the development of seed industry. Rice, corn, sorghum and other crops have already been hybridized, and they have been widely used in production one after another, which has greatly increased the yield. The applicant pinned the breakthrough in wheat breeding on the utilization of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12N15/82C12N15/65
Inventor 李中安
Owner NORTHWEST A & F UNIV
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