Method and its reagent unit for separating and purifying tumor cell

Abstract

A kind of reagent box which used to separate and purify tumor cells includes digesting agent for preparing suspending liquid of tumor cells, suspending agent for suspension of tumor cells, separating agent which is in separating vessel for separating and purifying tumor cells and cell filter for over-digested cells. The components of the digesting agent mentioned above were trypsase, collagenase I, collagenase IV or their mixture. The suspending agent includes buffering agent to stabilize the pH value of the circumstance for cells. The specific weight of the separating agent was between 1.020 and 1.025. It contained inorganic salt, amino acids, vitamins and cell factors. The separating vessel had test-tubes/centrifugal tubes with volumes between 15 and 50ml.

Description

technical field [0001] The invention relates to a method and kit for separating and purifying tumor cells. In particular, it relates to a separation and purification method for tumor cells in tumor pathological tissue blocks, malignant pleural effusion, ascites and other body cavity fluids, and cerebral effusions, and a kit for realizing the method. Background technique [0002] Obtaining purified tumor cells is very important for molecular biology research, disease mechanism research and disease treatment research of tumor diseases. However, because tumor cells are always associated with non-tumor cells such as lymphocytes, fibroblasts, stromal cells and tumor necrosis cells, it is difficult to obtain high-purity tumor cells by common cell separation methods. [0003] There have been public reports about the separation and purification of tumor cells in the prior art, such as Ma Jiyong, Wang Mingli, etc. "Journal of Anhui Medical University", 2002, 37 (2), pages 132-134, w...

AI Technical Summary

Problems solved by technology

The applicability of the above two methods is greatly reduced because the former requires the use of additional antibodies, while the latter is only practical for the isolation of a small number of tumor cells because it takes a long time and the process is difficult to control

In addition, Chinese patent application 03146933.7 discloses human serum and fetal bovine serum as separation and purification media. Since the use of serum for separation and purification involves safety issues for users, such as residual viruses in serum or cross-contamination, etc.