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Biological microcapsulation

A technology of microencapsulation and microencapsulation, which is applied in biochemical equipment and methods, immobilized enzymes, immobilized on/in organic carriers, etc. Short half-life and other problems, to achieve the effect of excellent fixation effect, excellent sustained release performance, and high mechanical strength

Inactive Publication Date: 2006-11-01
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its slow-release performance is usually poor, and the half-life of the capsule is short, which is not suitable for long-term use, which greatly limits the application prospects of microcapsules

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Micrococcus sp. liquid seed preparation

[0021] Pick up 2 rings of Micrococcus sp. lawns on the solid base medium with an inoculation loop, insert them into the seed medium, and cultivate them for 16-20 hours at 130rpm-140rpm and 28°C-30°C to obtain liquid seeds;

[0022] [Seed medium] Weight composition: 1.25% glucose, 0.25% yeast extract, 0.1% NH 4 NO 3 , 0.02% MgSO 4 ·7H 2 O, 0.02% KCl, pH=7.0-7.2;

[0023] (2) wall material solution preparation (mass percentage)

[0024] Prepare 2.0% sodium alginate aqueous solution, sterilize with damp heat at 111°C for 30 minutes, cool to 45°C under aseptic conditions, add the above-mentioned 20% total mass of Micrococcus sp. liquid seeds, and stir evenly;

[0025] (3) Preparation of a complex coagulation core material solution (mass percentage)

[0026] Prepare a mixed aqueous solution of 2.5% gelatin and 1.6% calcium chloride, adjust the pH to 5.5, and sterilize with damp heat at 111°C for 30 minutes;

[0027] (4) se...

Embodiment 2

[0038] The difference from Example 1 is:

[0039] When preparing the primary complex coacervation core material solution, adjust the pH=6.5; when preparing the secondary complex coacervation core material solution, adjust the pH=4.5; the weight loss rate of the microcapsules prepared by the above steps is less than 25.1% in 4 weeks.

Embodiment 3

[0041] The difference from Example 1 is:

[0042] When preparing the primary complex coacervation core material solution, adjust the pH=6.3; when preparing the secondary complex coacervation core material solution, adjust the pH=4.0; the weight loss rate of the microcapsules prepared by the above steps is less than 19.4% in 4 weeks.

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PUM

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Abstract

A biological and micro-capsule technology to improve slow-release capability of micro-capsule which includes the following steps: add a strain of microorganism liquid seed into sodium alginate solution ,add the above solution into glutin-CaCl2 intermixture with certain pH value in 40-45DEG C temperature and 40-60 drops / min velocity, stabilize for3.5-5h to form micro-capsule rudiment, add CaCl2 solution to re-calcify for 10-15min, add chitosan solution with certain pH value for 2-3h for re-coacervation, immerse in steriled saline solution for 6-24h for proliferating. Micro-capsule made by this method shows uniform shape, well fastness and excellent slow-release capability.

Description

technical field [0001] The invention relates to a biological method of cell immobilization, especially refers to the microencapsulation process of sodium alginate (Na Alg)-chitosan (CS)-gelatin (Gel) controlled by mediating pH value, so as to make the microcapsules immobilize the activity of microorganisms Maintenance and sustained-release performance maintenance, specifically a biomicroencapsulation method that can improve the sustained-release performance of biomicrocapsules. Background technique [0002] Microencapsulation technology is a bead-shaped, ellipsoid-shaped or ellipsoid-shaped microcapsule formed by encapsulating enzymes, coenzymes, proteins and other biological macromolecules or microorganisms, animal and plant cells in a hydrophilic semi-permeable membrane. Biomacromolecules and cells are blocked inside or outside the membrane of the microcapsule, while oxygen, nutrients and other small molecules can freely pass through the microcapsule membrane for material ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/00C12N11/04
Inventor 李海波李培军鞠京丽张海荣许华夏
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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