Cotton alpha-globulin promoter for seed-specific expression of transgenes

A technology of transgenic plants and genes, applied in genetic engineering, plant genetic improvement, angiosperms/flowering plants, etc., can solve unwise problems

Inactive Publication Date: 2006-11-29
TEXAS A&M UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if more than one gene needs to be expressed to the same extent, it is unwise to use the same promoter for multiple introduced genes

Method used

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  • Cotton alpha-globulin promoter for seed-specific expression of transgenes
  • Cotton alpha-globulin promoter for seed-specific expression of transgenes
  • Cotton alpha-globulin promoter for seed-specific expression of transgenes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0052] figure 1 The sequence of the α-globin promoter and the construct of the reporter gene are shown. figure 1 A (SEQ ID NO. 1) shows the 1144 bp sequence isolated and functionally characterized in this study. Putative cis-acting elements are shown in boxes. Transcription start sites are indicated with +1. The 5' untranslated region is in italics and an additional unpublished 336 bp sequence is underlined. The 336bp sequence is shown in Figure 10 (SEQ ID NO.3). The 5' flanking promoter region was cloned using sequence information from an alpha-globin clone from a cotton staged embryo cDNA library according to the method described by Siebert et al. (1995). The 772 bp of this clone towards the 3' end of the sequence shown here matched the published 5' genomic flanking sequence of the alpha-globin gene B (Chlan et al., 1987). A further PCR walk yielded an additional 336 nucleotides of upstream sequence (underlined). Based on the promoter sequence information, primers we...

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PUM

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Abstract

The present invention is directed to 5' regulatory regions of a cotton seed-specific gene, alpha -globulin. The 5' regulatory region, or parts thereof, when operably linked to either the coding sequence of a native gene, heterologous gene or a sequence or complementary sequence in a plant seed. The regulatory regions are useful in expression cassettes and expression vectors for the trnasformation of plants. Also provided are methods of modulating the levels of a native or heterologous gene such as a fatty acid synthesis or lipid metabolism gene by transforming a plant with the subject expression cassettes and expression vectors.

Description

[0001] Inventors: Keerti S. Rathore, Ganesan Sunilkumar, James P. Connell and Avutu S. Reddy field of invention [0002] The present invention is in the field of transgene expression. More specifically, the present invention is in the field of gene transcription promoter elements for transgene expression in plants. Background of the invention [0003] Seed-specific transgene expression is required for many applications using genetic engineering. These applications include transgenic approaches to improve the nutritional quality of seeds by manipulating flux through metabolic pathways (Hitz et al., 1995; Kinney, 1996; Shintani and DellaPenna, 1998; Goto et al., 1999) and the production of seeds with industrial or new compounds of medicinal value (Cahoon et al., 2000). Some of these transgenic traits may require expression of more than one transgene in developing seeds (Ye et al., 2000). In other cases, metabolic engineering to improve seed quality may require the overexpre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/29C12N5/10A01H5/00A01H5/10
CPCC12N15/8234
Inventor K·S·拉瑟尔G·苏尼库玛J·P·康奈尔A·S·雷迪
Owner TEXAS A&M UNIVERSITY
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