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Application of tectori-genin in preparation of medicine for preventing and treating liver disease and liver cancer

A technology for irisin and liver disease, applied in the field of irisin, can solve problems such as no reports on the prevention and treatment of liver disease and anti-cancer of iris.

Inactive Publication Date: 2006-12-20
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Irisgenin, full name 5,7,4'-trihydroxy-6-methoxy isoflavone, also known as irisaxanthin, English name is Tectorigenin (hereinafter referred to as Tec), its structural formula is as follows, is a class of polyhydroxy Isoflavones, which are widely distributed in Iridaceae plants, have antioxidant and estrogen-like biological activities, but there are no reports on the prevention and treatment of liver diseases and anti-cancer by iris

Method used

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  • Application of tectori-genin in preparation of medicine for preventing and treating liver disease and liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Example 1: Preparation of iris aglycone Take 100 grams of dried iris rhizome powder, extract with 90% ethanol under reflux, and concentrate the extract under reduced pressure until it has no alcohol smell (about 70 ml). The concentrate was rehydrated to 400ml, and sodium bicarbonate was added thereto to make the final concentration of sodium bicarbonate 5%. After fully stirring in a water bath at 80°C for half an hour, a clear filtrate was obtained by filtration. Concentrated hydrochloric acid was added to the filtrate to make the pH of the filtrate 2-3, and the precipitate was filtered out (dry weight: 8.7 g). Use 800ml of ethyl acetate to fully dissolve the eluate in a water bath at 60°C, and filter off the insoluble matter to obtain a clear ethyl acetate solution. First extract the ethyl acetate solution twice with 5% sodium bicarbonate solution of equal volume (800ml), discard the sodium bicarbonate solution, then extract the ethyl acetate solution once with 2% sod...

Embodiment 2

[0012] Example 2. The effect of irisin (Tec) on preventing and treating liver damage

[0013] (1) Protective effect of Tec on D-galactosamine (D-GalN)-induced acute liver injury in mice

[0014] The mice were divided into 5 groups, and the control group was intragastrically administered with normal saline (0.3 mL·d -1 ), for 7 consecutive days, on the 7th day intraperitoneal injection of normal saline (0.15mL·10g -1 ); D-GalN, Tec (15, 30, 60 mg·kg -1 ) Each group was given intragastric administration of normal saline and different concentrations of Tec (0.3mL·d -1 ), for 7 consecutive days, on the 7th day intraperitoneal injection of D-GalN (800mg·kg -1 , 0.15mL·10g -1 ), 16 hours later, blood was taken to measure AST and ALT activities, and the results are shown in Table 1.

[0015] It can be seen from Table 1 that Tec can effectively inhibit the significant increase of AST and ALT activities in mice caused by D-GalN in a dose-dependent manner.

[0016] group ...

Embodiment 3

[0033] Example 3. The antitumor effect of irisin

[0034] 1. MTT method to test anti-tumor activity in vitro

[0035] Cell incubation: Take tumor cells in the logarithmic growth phase and adjust the concentration of the cell suspension to 1-1.5×10 5 ml -1 . Add 100 μl of cell suspension to each well of a 96-well culture plate, and culture in a 37° C., 5% CO2 incubator for 24 hours. After culturing for 24 hours, the medicinal solution was added according to the design.

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PUM

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Abstract

The irigenin has protective function on the mouse instant liver hurt induced by D-galactosamine,D-GalN, has protective function on the mouse instant liver hurt induced by CC14, has cure function on big mouse liver fiber caused by composite factors, and high restrain function on the liver cancer cell. Therefore, irigenin can be used to prepare relative medicine.

Description

technical field [0001] The present invention relates to irisin, and in particular relates to irisin as medicine for preventing and treating liver disease or liver cancer. technical background [0002] Irisgenin, full name 5,7,4'-trihydroxy-6-methoxy isoflavone, also known as irisaxanthin, English name is Tectorigenin (hereinafter referred to as Tec), its structural formula is as follows, is a class of polyhydroxy Isoflavones, which are widely distributed in Iridaceae plants, have antioxidant and estrogen-like biological activities, but there are no reports on the prevention and treatment of liver diseases and anti-cancer by iris. [0003] [0004] Tectorigenin (Tectorigenin, 5,7,4'-trihydroxy-6-methoxyisoflavone) Contents of the invention [0005] The present inventor has carried out pharmacological research to iris aglycone, and finds that iris aglycone has protective effect on mouse acute liver injury induced by D-galactosamine (D-galactosamine, D-GalN), and has a pr...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P1/16A61P31/14A61P31/20A61P35/00C07D311/36
Inventor 谭仁祥吴俊华徐琛陈运喜李方李洪森周添
Owner NANJING UNIV
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