Process for extracting high-content hypericin from Hypericum perforatum L.

A technology of Hypericum perforatum and hypericin, applied in organic chemistry, quinone separation/purification, etc., to achieve obvious growth inhibition effect and simple process effect

Inactive Publication Date: 2007-04-25
SHENZHEN POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

But the products of above these methods gained can only reach the basic requirement of the hypericin content ≥ 0.3% required on the international market, there is no report yet for extracting and purifying the production higher hypericin concentration extract from natural medicine

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  • Process for extracting high-content hypericin from Hypericum perforatum L.

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Embodiment Construction

[0020] As shown in Figure 1, the present invention provides a kind of extraction method of hypericin extract in Hypericum perforatum, comprises the steps:

[0021] A, preparation step of hypericin silica gel column chromatography extract

[0022] A1. Prepare samples

[0023] Take 1 to 3 parts by weight of Hypericum perforatum crude extract, add 18 to 22 parts by volume of methanol to dissolve, ultrasonicate for 20 to 40 minutes, and filter; weigh 1 to 3 parts by weight of column chromatography silica gel (200 to 300 mesh), add the above treatment The Hypericum perforatum extract was mixed evenly, and the solvent was evaporated to dryness by a rotary evaporator.

[0024] A2. Preparation of eluent

[0025] According to the optimized results of thin layer chromatography (TLC), this experiment adopts chloroform-methanol gradient elution, and the polarity of the eluent gradually increases, chloroform:methanol=9:1-8.5:1.5-8:2 -7.5:2.5 (v / v).

[0026] A3, column packing

[0027]...

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Abstract

The invention discloses an extracting method of hypericin extract from Hypericum, which comprises the following steps: A1. Preparing sample; A2. Allocating eluent; A3. Loading column; A4. Sampling; A5. Eluting silica gel column; A6. Collecting group by group; B1. Predisposing gel; loading column; B2 sampling; B3. Eluting; collecting to obtain the product. The invention simplifies the technique with high extracting rate, which inhibits HepG2, A549, SpcA1 and MDA231 from growing effectively.

Description

technical field [0001] The invention relates to a method for extracting hypericin extract, in particular to a method for extracting hypericin extract from Hypericum perforatum. Background technique [0002] Hypericum perforatum (Hypericum performatum L. or St. John's wort), also known as Hypericum perforatum, is a perennial herb of the genus Hypericum in the family Garcinia. Traditional Chinese medicine believes that it has astringent, hemostatic and antibacterial effects, and it has a long history of being used in Europe to treat depression and other diseases. Hypericin, a naphthalene dianthrone substance in Hypericum perforatum, has a variety of physiological activities. In antiviral aspects, it can inhibit a variety of retroviruses, including human immunodeficiency virus (HIV) and bovine immunodeficiency virus (BIV). , murine leukemia virus (LP-BM5) and highly pathogenic avian influenza virus (H5N1 and H9N2), as well as a variety of enveloped viruses, such as hepatitis B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C50/36C07C46/10
Inventor 张俊松王晓利
Owner SHENZHEN POLYTECHNIC
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