The invention discloses a
hypericum perforatum extract insecticide for preventing and treating
plutella xylostella and application of the
hypericum perforatum extract insecticide, and belongs to the technical field of
agriculture. Firstly, a
hypericum perforatum
ethanol crude extract is prepared and extracted with
ethyl acetate to obtain an
ethyl acetate extraction fraction with the effect of preventing and treating
plutella xylostella; furthermore, on the basis of the biological affinity effect of the acetylcholin
esterase, the
trypsin and
small molecule ligands in the extraction fractions of the
ethyl acetate, insecticidal active compounds are screened, and the screened multiple
active components have potential acetylcholin
esterase and
trypsin multi-target inhibitory activity. Animal experiment results show that the botanical insecticide prepared by taking
luteolin-7-O-
glucoside,
quercetin or ethyl acetate extraction fractions as main functional components has an excellent
plutella xylostella prevention and
treatment effect, is environment-friendly and does not generate secondary
pollution. The invention provides an important strategy for the development of biopesticides taking acetylcholin
esterase and
trypsin as multiple targets.