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Discrimination antiserum/antibody preparation and its preparation method and use

An antiserum and discriminating technology, applied in the field of discriminative antiserum/antibody preparations and their preparation, and antiserum antibody/antibody preparations, can solve the problems of small detection throughput, poor sensitivity and time-consuming, and achieve high sensitivity and specificity. The effect of strong performance and simple preparation and screening method

Active Publication Date: 2011-09-14
西安血氧生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The object of the present invention is to provide a kind of differential antiserum / antibody preparation and its preparation method and application, which solves the technical problems of poor sensitivity, low detection throughput, time-consuming, inability to judge and differentiate hemoglobin from different sources in the background technology

Method used

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Embodiment Construction

[0038] The present invention uses antibodies produced by different animals to heterologous and highly conserved proteins with very similar structures, including polyclonal and monoclonal antibodies, to identify corresponding proteins from different sources. First, prepare antiserum antibodies that can identify different types of heterologous hemoglobin in biological samples; then use these antiserum antibodies to identify heterologous hemoglobin in biological samples through specific antigen-antibody binding reactions, and obtain specific identification It is a highly sensitive antiserum for various immunoassays with high sensitivity, rapidity and large throughput.

[0039] For example: hemoglobin is a rare and highly conserved protein in the animal world, but there are also very subtle changes in amino acid sequence or spatial microstructure in hemoglobin among different animals. When a certain animal is stimulated with hemoglobin from a certain source, the antibodies produce...

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Abstract

A preparation of discrimination antiserum / antibody is prepared by selecting out said preparation after using hemoglobin from certain source and antiserum generated by immune-irritating human or animal to carry out combination reaction with antigen-antibody of multiple animal hemoglobin, utilizing specific antigen-antibody reaction to only discriminate hemoglobin generated by irritation or / and to discriminate hemoglobin of the other different animal variety.

Description

technical field [0001] The present invention relates to an antiserum / antibody preparation capable of differentiating hemoglobin from different animal sources in a sample, a method for preparing such a discriminating antiserum / antibody preparation, and a method for obtaining specific and discriminative antiserum / antibody preparations through a specific antigen-antibody binding reaction. Antiserum antibodies / antibody preparations. The differential antiserum / antibody preparation is used to identify or qualitatively and quantitatively measure specific animal species hemoglobin in biological samples, and is used for various immunological detections with high sensitivity, rapidity and large throughput. It specifically relates to a differential antiserum / antibody preparation and its preparation method and application. Background technique [0002] Hemoglobin is a highly conserved protein. The identity of amino acid sequences of hemoglobin of various animals is very high. For exam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N33/531
Inventor 但宁王童文王丽莉
Owner 西安血氧生物技术有限公司