Bifidobacterium longum cbt bg7 strain for promotion of growth and nutraceutical composition for promotion of growth containing the same
a technology of bifidobacterium longum and cbt bg7, which is applied in the direction of microorganisms, biochemical apparatus and processes, bacteria based processes, etc., can solve the problems of low growth promotion effect and high cost of growth hormone use, and achieve excellent growth promotion effect, promote vitamin biosynthesis, and difficult to industrialize
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example 1
Isolation and Identification of Bifidobacterium longum CBT BG7 Strain
[0039]1-1: Selection of Novel Strain
[0040]1 g of human feces was serially diluted in sterile anaerobic water, and 1 ml of each of the dilutions was poured on glucose blood liver (BL. BD. USA) solid medium and cultured for 3 days under anaerobic conditions. The produced colonies were selected using a Bifidobacterium selective medium obtained by adding BCP (Bromocresol purple, 0.17 g / L) to BL, and then cultured for 3 days under the same conditions. Bifidobacterium colonies, the color around which changed to yellow, were selected, and then biochemical and molecular biological identification of the selected colonies was performed. Thereafter, one strain having the best physical properties was selected.
[0041]1-2: Identification of Selected Strain
[0042]1) Analysis of Sugar Utilization
[0043]The sugar utilization of the strain isolated from human feces was examined using an API 50 CHL kit. The results obtained by examining...
example 2
Analysis of Genes of Bifidobacterium longum CBT BG7 Strain
[0072]Genome sequencing of the Bifidobacterium longum CBT BG7 strain (KCTC 12200BP) was performed using a PacBio RS II System (DNA Link, Republic of Korea). For the genome of the strain, a 10-kb library was constructed, and genome sequencing was performed using one of SMRT cells with C2-P4 chemistry. By the genome sequencing, a sequence having a length of 337,655,282 bp was obtained. De novo assembly was performed using SMRTpipe HGAP, and scaffolding and gap filling were performed using SMRTpipe AHA. Prediction of structural genes was performed using Glimmer3, and gene annotation was performed by AutoFACT (Koski et al. 2005) using the results obtained by BLASTP for Pfam, Uniref100, KEGG, COG and GenBank NR databases. Transfer RNA and ribosomal RNA were performed using tRNAscan-SE (Lowe and Eddy 1997) and RNAmmer (Lagesen et al. 2007), respectively. The functional classification of genes by Clusters of Orthologous Groups (COGs...
example 3
Growth Promoting Effect of Bifidobacterium longum CBT BG7 Strain
[0083]The following Example demonstrates the characteristics and growth promoting effect of the strain of the present invention. All the experimental results obtained in the Example were expressed as mean±SD, and statistical processing of the experimental results was performed using GraphPad Prism™ 6.0. In addition, the significance of difference in means between the test groups was determined by one-way ANOVA, and then the Post Hoc test was performed using Tukey's multiple range test.
[0084]3-1: Preparation of Culture of Strain of the Present Invention and Composition Containing the Same
[0085]The Bifidobacterium longum CBT BG7 strain (KCTC 12200BP) was cultured in BL broth (BD Diagnostics, Sparks, MD) at 37° C. for 24 hours, and diluted to 1011 CFU / ml in phosphate buffered saline (PBS, 10 mM sodium phosphate, 130 mM sodium chloride, pH 7.4). The dilution was sonicated and centrifuged to separate the supernatant, followe...
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