Methods of diagnosing cardiovascular disease
a cardiovascular disease and disease technology, applied in biochemistry apparatus and processes, organic chemistry, fermentation, etc., can solve the problems of cardiovascular disease risk and increased risk of cardiovascular disease, and achieve the effect of increasing the risk of cardiovascular diseas
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
example 1
Determination of the Haplotype Structure of the CD36 Locus
[0110]To determine the haplotype structure of the CD36 locus in Caucasians, 21 common SNPs (frequency≧0.05) were typed in 72 unrelated individuals from the general population. Study subjects were genotyped at polymorphic loci by means of PCR followed by dot blotting and allelic specific hybridization or single base extension / fluorescence polarization (AcycloPrime™-FP SNP Detection System) using a Wallac VICTOR2™ Multilabel Plate Reader (Perkin-Elmer).
[0111]Eight of the 21 SNPs were representative of the major linkage disequilibrium bins identified in the region between intron 3 and exon 14 by a resequencing project (available at pga.gs.washington.edu / data / cd36 / ). The other eleven were selected from the dbSNP database to cover the two alternative promoters (Sato et al., J. Biol. Chem., 277:15703-11 (2002)), the 5′ non-coding exons, and the 3′ UTR in exon 15. The 21 SNPs spanned a total of 63 Kb, resulting in a 3.0 Kb average s...
example 2
Demonstration of an Association Between FFA Levels and CD36 SNPs
[0114]The association between CD36 variants and metabolic traits was evaluated in 585 Caucasian residents of the Gargano area (East Coast of Italy). Their clinical characteristics are reported in Table 1. Subjects were recruited among the employees of the hospital ‘Casa Sollievo della Sofferenza’ (San Giovanni Rotondo, Italy), who had fasting plasma glucose Stress testing. Principles and Practice, F A Davis, Philadelphia. (1986)). The inter-and intra-assay coefficients of variation were 2.2% and 2.8%, respectively.
[0115]Continuous variables were compared among genotype groups by ANOVA using the PROC GLM procedure of the SAS software package (SAS Institute, Cary, N.C.). All analyses included gender and age as covariates. Fasting insulin, triglycerides, and FFA were analyzed after logarithmic transformation. The association between FFA levels and common (≧0.05) CD36 haplotypes was analyzed using the score statistics propo...
example 3
Demonstration of an Association Between CAD and CD36 SNPs
[0121]To determine whether the CD36 AAGIC haplotype was also associated with coronary artery disease (CAD), two populations of individuals with type 2 diabetes (defined according to the WHO criteria) were studied, one from Boston (n=197), the other from San Giovanni Rotondo, Italy (n=321). The study protocol and informed consent procedures were approved by the local research ethic committees. Each population included a group of CAD-positive cases and a group of CAD-negative controls. In the Boston study, the CAD-positive cases, defined as subjects who had a stenosis greater than 50% in at least one major coronary artery or their main branches, were recruited among type 2 diabetic patients who underwent cardiac catheterization at the Beth Israel Deaconess Medical Center (BIDMC) between Feb. 1, 2000, and Jan. 31, 2002. CAD-negative controls were Joslin patients (the Joslin Clinic serves as the BIDMC Diabetes Clinic) who were age...
PUM
| Property | Measurement | Unit |
|---|---|---|
| temperature gradient gel electrophoresis | aaaaa | aaaaa |
| size analysis | aaaaa | aaaaa |
| insulin-resistance | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More