Method to detect virus related immunological markers for the diagnosis of respiratory tract infections

a technology of respiratory tract infections and immunological markers, which is applied in the direction of biochemistry equipment, biochemistry equipment and processes, material testing goods, etc., can solve the problem of limited throughpu

Inactive Publication Date: 2012-04-17
CMED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The patent text describes a technology called surface plasmon resonance (SPR) that can be used to detect and analyze biomolecule interactions in real-time. This technology offers several advantages over other methods, such as fluorescence or ELISA, and can be used to detect virus-related immunological markers in blood for the diagnosis of respiratory tract infections. The text also describes the development of a high-throughput SPR instrument that can measure binding events of biomolecules at a metal surface by detecting changes in the local refractive index. The SPR technology can be used to study interactions between immobilized biomolecules and a solution-phase analyte, and has been shown to be a powerful tool for studying biomolecule interactions."

Problems solved by technology

Traditional SPR spectroscopy sensors, which measure the entire SPR curve as a function of angle or wavelength, have been widely used, but offer limited throughput.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0057]Detection of respiratory tract viruses-related immunological markers in blood for the diagnosis of respiratory tract infections.[0058](A) Testing sample: serum (about 2 ml)[0059](B) Representative antigens used to detect the respective respiratory tract viruses-related immunological markers (antibodies) in a serum sample: agents of human parainfluenza virus 1, human parainfluenza virus 2, human parainfluenza virus 3, respiratory syncytial virus, influenza virus A, influenza virus B, and adenovirus.[0060](C) Procedure:

[0061]Step One: Formation of a Linking Layer on The Surface of a Gold-film Glass Chip:

[0062]1. Cleanliness of Substrate

[0063]Metal substrates (copper, silver, aluminum or gold) were firstly cleaned with strong oxidizing chemicals (“piranha” solution-H2SO4:H2O2) or argon plasmas, then the surfaces of these substrates were washed with ultra pure water and degassed ethanol. After rinsing, the substrates were dried with pure N2 gas stream.

[0064]2. Preparation of Self-...

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Abstract

This invention discloses using SPR technology to simultaneously and qualitatively detect the presence of respiratory tract viruses-related immunological markers in a serum sample, which can be used for the diagnosis of respiratory tract infections. It also discloses an efficient formula to make a mixed SAM that can greatly enhance the immobilization ability of the metal surface in SPR based techniques, which is good for the immobilization of representative antigens used to detect the respective respiratory tract viruses-related immunological markers (antibodies) in blood for the diagnosis of respiratory tract infections.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority benefits under 35 U.S.C. 365(a) of International Application No. PCT / US07 / 78229, filed 12 Sep. 2007, which claims priority under the Paris Convention to U.S. Provisional Patent Application No. 60 / 827,187, filed on 27 Sep. 2006, which applications are incorporated by reference herein in their entireties, including but not limited to those portions that specifically appear hereinafter.TECHNICAL FIELD[0002]The present invention relates to a novel method of using SPR technology to simultaneously detect the presence of respiratory tract viruses-related immunological markers in blood for the diagnosis of respiratory tract infections.INDUSTRIAL APPLICABILITY[0003]It has been recognized that it would be advantageous to develop a label-free and high-throughput technique to simultaneously detect the presence of respiratory tract viruses-related immunological markers in blood for the diagnosis of respiratory tract in...

Claims

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Application Information

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Patent Type & AuthorityPatents(United States)
IPC IPC(8): G01N33/569G01N33/553
CPCB01J19/0046G01N33/54353G01N33/54373G01N33/56983B01J2219/00527B01J2219/00605B01J2219/00659B01J2219/00702B01J2219/00722B01J2219/00725B01J2219/0074G01N2800/12Y10S435/808Y10S436/805
InventorCHEN, ZHONGLIU, NINGLI, YANCUN
OwnerCMED TECH