Concentration measuring method, concentration test instrument, and concentration measuring apparatus
a technology of concentration measurement and test instrument, which is applied in the direction of instruments, measurement devices, scientific instruments, etc., can solve the problems of blood sugar not being accurately measured, the effect of reducing substances, and the measurement error is more pronounced, so as to reduce the effect of background current, and improve the effect of accuracy
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
example 1
[0091]In this example, the electrode response characteristics of glucose sensors were evaluated by examining CV waveforms. The CV waveform was examined by applying spots of glucose solution on the reagent layer of the glucose sensor, sweeping such that the sweep rate was 50 mV / sec and the applied voltage was 0 mV→+800 mV→0 mV→−800 mV→0 mV→+800 mV, and measuring the response current during the sweep. The glucose solution used here was a standard solution with a concentration of 200 mg / dL (prepared by dissolving glucose in physiological saline (0.9 wt % NaCl)). The amount of spot application of the glucose solution on the reagent layer was 1 μL. FIG. 6 shows the CV waveforms.
[0092]It can be seen from the CV waveforms in FIG. 6 that within a range in which the applied voltage was 0 mV→+800 mV on the second time, the response current value was at its maximum when the applied voltage was approximately 100 mV with the glucose sensor 1 of the present invention in which [Ru(III)(NH3)6]Cl3 w...
example 2
[0094]In this example, it was examined whether glucose concentration can be accurately measured at a low voltage (200 mV). To this end, the response current value was measured using four different standard solutions with glucose concentrations of 0 mg / dL, 200 mg / dL, 400 mg / dL, and 600 mg / dL and using the glucose sensor 1 of the present invention and the comparative glucose sensor 1, at applied voltages of 500 mV and 200 mV. The response current value was measured 5 seconds after the spot application of 1 μL of standard solution to the reagent layer, with the application of voltage held steady between the first and second electrodes. These results are given in FIG. 7.
[0095]As can be seen from FIG. 7. when the applied voltage was 500 mV, the glucose sensor 1 of the present invention exhibited good linearity for the group of plotted points, indicating that glucose sensor can be measured favorably even when the glucose concentration is high (400 mg / dL or higher). In contrast, with the c...
example 3
[0098]In this example, it was examined how long it took to measure the glucose concentration favorably. To this end, 500 mV voltage application between the first and second electrodes was commenced 0, 1, 2, or 10 seconds after the spot application of 1 μL of whole blood with a glucose concentration of 400 mg / dL to the reagent layer, the response current was measured during sustained voltage application, and the change over time was measured. These results are given in FIGS. 8 and 9.
[0099]It can be seen from FIG. 8 that with the glucose sensor 1 of the present invention, the individual measurement values obtained 3 seconds after the start of voltage application were the same, regardless of the time before voltage application (the time in a non-application state). Therefore, it can be concluded that the glucose sensor of the present invention gives stable measurement results as long as the application time is at least 3 seconds, and, as seen in FIG. 9, that the application time can be...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More