34 results about "Cancer Cellularity" patented technology

The invention discloses a liver cancer-specific gene-virus and application thereof. The virus is an oncolytic virus; an early gene E1 of the virus is divided into E1A and E1B; a promoter of the E1A is replaced by a liver cancer-specific promoter, and is preferably replaced by a liver cancer-specific alpha fetoprotein promoter; and an anticancer gene is carried by the virus. The anticancer gene is SOCS3, HCCS-1, TSLC-1, 1L-24, MnSOD or TRAIL. The anticancer gene is preferably IL-24. The liver cancer-specific gene-virus is used for specific medicines for treating liver cancer, has a relatively high targeting anti-hepatoma effect, basically does not affect normal cells, and also has a small effect on most of other cancer cells.

The invention discloses a preparation method of a folic acid self-assembled water-soluble rare-earth doped up-converted nanometer material. The preparation method mainly comprises the following steps: taking 9mL of diglycol and 6mL of high-purity water as solvents, adding 1mmol of Ln(Y / Yb / Er=80 / 18 / 2), 16mmol of NaF and 0.15g of polyethyleneimine (PEI), measuring the pH which is 9, stirring and reacting for 0.5 hour in the presence of nitrogen at 200 DEG C, transferring to a reaction kettle, reacting for 24 hours at 200 DEG C, naturally cooling and centrifugally washing to obtain polyethyleneimine modified up-converted nanometer particles (PEI-UCNPs), dispersing 1g of the PEI-UCNPs in the high-purity water, adding 0.1g of folic acid, oscillating for 15 minutes, centrifugally washing, and then dispersing in the high-purity water to obtain folic acid self-assembled up-converted nanometer particles (FA-UCNPs). As the folic acid and a folic acid receptor can be specifically bound with each other, the folic acid self-assembled rare-earth doped up-converted nanometer material can be used for identifying cancer cells and applied to targeting imaging of the cancer cells.

The invention discloses a cancer cell line therapeutic drug prediction method based on multidimensional network, which mainly solves the problem that the prior art has low accuracy of cancer cell linetherapeutic drug prediction results. The solution is to download data of n cancer cell lines to construct a similarity network of the cancer cell lines; download data of m drugs to construct a drug similarity network; calculate a low-dimensional eigenvector matrix of the n cancer cell lines by using a diffusion component analysis algorithm in the similarity network of cancer cell lines; calculatea low-dimensional eigenvector matrix of the m drugs by the diffusion component analysis algorithm in the drug similarity network; obtain a logistic regression model of drug response; calculate the score of the sensitivity relationship between the drugs and the cancer cell lines; and judge whether the drug has a therapeutic effect on the cancer cell line by using the score of the sensitivity relationship. The invention improves the accuracy of cancer cell line therapeutic drug prediction results, and can be used for the prediction experiment of the cancer response to the drug.

The invention discloses a method for evaluating an anti-cancer drug inhibiting the aggregation of membrane protein receptors. The method comprises the following steps of: (1) conveying genes of target membrane protein receptors to an excised cancer cell, and marking the genes of the target membrane protein receptors by fluorescent protein to obtain a reconstructed cancer cell; (2) hatching the reconstructed cancer cell and a substance to be evaluated, then hatching by adding ligands of the target membrane protein receptors after the last hatching is finished, and then fixing the reconstructed cancer cell by adding a PBS (Phosphate Buffered Saline) solution of paraformaldehyde to obtain a cell sample I to be detected; and (3) carrying out fluorescence imaging on the unimolecule of the cell sample I to be detected to obtain a cell image and furthermore obtain bleaching steps of each fluorescent spot, and analyzing the image and counting the bleaching steps of the fluorescent spot to judge whether the substance to be evaluated plays an inhibition role in the aggregation of the membrane protein receptors or not. The method has the advantages of simpleness and convenience for operation, short evaluation period and low expense and can be used for evaluating various drugs inhibiting the aggregation of membrane protein receptors.

The invention provides a botanical drug active part of aquilaria sinensis (Lour.) Spreng., a preparation method thereof, a pharmaceutical composition composed of the active part, and application of the botanical drug active part and the pharmaceutical composition in preparation of drugs for treating tumors or cancers. The preparation method comprises the following steps: extracting and extracting dried flos aquilaria sinensis by using a solvent to prepare PXS65, PXS66, PXS66-1, PXS66-2 and PXS66-3; the pharmaceutical composition has remarkable inhibitory activity on cancer cell strains, such as lung cancer SPC-A-1 cells, lung cancer NCI-H520 cells, lung cancer A549 cells, cervical cancer HeLa cells, human neuroblastoma SH-SY5Y cells, ovarian cancer SK-OV-3 cells, leukemia MT4 cells, prostate cancer PC-3 cells, liver cancer SMMC-7721 cells, breast cancer MDA-MB-231 cells, lung cancer NCI-H446 cells, lung cancer taxol drug-resistant strain A549 / Taxol cells and the like. The flos aquilaria sinensis extract disclosed by the invention provides a new choice for drugs for treating or preventing tumors or cancers and drugs for improving tumor or cancer drug resistance.

The invention relates to the field of biological medicine, in particular to a method for reprogramming cancer cells into non-cancer cells. The invention particularly provides a mixture (cocktail) andmethod for reprogramming cancer cells into non-cancer cells. In the method, the reprogramming mixture is applied to a tumor site and used for directly reprogramming malignant cells into non-malignantcells, more particularly, a packaged (encapsulated) reprogramming mixture is applied to a tumor site in a test person, particularly, is applied to glioblastoma multiforme (GBM) so that the GBM can beinduced to become non-malignant nerve cells and / or glial cells. The invention also relates to a method for preparing the mixture, such as, a method for preparing the reprogramming mixture containing one ore more protein kinase inhibitors.

The invention relates to the technical field of biomedicines, in particular to an NKT (Natural Killer T) cell preparation prepared by sensitization by using tumor cells and simulating factors and a preparation method of the NKT cell preparation. The NKT cell preparation is high in killing activity and can effectively kill remaining cancer cells, eliminate recurrence and metastasis factors, increase the curability, prolong the survival time of patients and improve the life quality by applying an immunotherapy. In the preparation process of the preparation, NKT cells are jointly cultured by adopting IL-2, IL-15 and IL-21 and sensitized by adopting the tumor cells and the stimulating factors, so that the expansion effects of the NKT cells are enhanced, and the killing activity of the NKT cell preparation is also greatly enhanced.

The invention discloses an application of a piperazine derivative as a p53 molecule activity regulator. Mouse and human cell experimental results show that the piperazine derivative can regulate expression of p53 downstream genes, also can make growth and proliferation of normal cells maintained and make proliferation of cancer cells inhibited, and has wide application prospects in the field of cancer therapy. In addition, through regulation of p53 molecules, the piperazine derivative also can adjust a pluripotency signal network of stem cells, inhibit differentiation of the stem cells or maintain the pluripotency of the stem cells, thereby improving the line establishment efficiency of the pluripotent stem cells.

At present, the cancer treatment methods include the operative treatment, the radioactive treatment and the chemical treatment. Great side effects are brought to a patient during treatment with the radioactive treatment and the chemical treatment. According to the methods, huge injuries to normal cells are generated while the cancer cell development is choked. According to a whole-body tepidity cancer rehabilitation method, the in vivo temperature rapidly rises in a short time to enable the normal cells to be most active and to enable cancer cells to be difficult to survive, so that the function of improving autoimmunity is achieved, and the human body returns to the immunity level of a normal person.

The invention discloses multifunctional jerusalem artichoke capsules for treating diabetes by nourishing pancreas, eliminating root causes and suppressing cancer. The capsules comprise the following basic components by weight: 2g of jerusalem artichoke powder, 2g of lychee seed, 2g of longan seed, 1.5g of pawpaw seed, 1.5g of lalang grass rhizome, 1.5g of bitter gourd, 1g of eel, 0.5g of manyflower solomonseal rhizome and 0.5g of yam. Through mutual compatibility effect of the raw materials, the functions of spleen and stomach can be adjusted, the immunity of body is improved, and directional nutrient components such as protodioscin of the lychee seed, saponin fat and tannin of the longan seed, ferment of the pawpaw seed and the like can be utilized to nourish the pancreas and eliminate the root causes so as to fulfill the aim of suppressing and lowering glucose; and the saponin and alkaloid component included in the capsules can greatly suppress and damage mitosis of cancer cells, so that the cancer cells cannot divide but even die. The used raw materials have wide sources and low cost and do not have side effect on human bodies.

Antibodies that specifically bind to CD38, as well as constructs comprising such antibodies fused to attenuated interferon alpha-2B proteins are provided. Anti-CD38-attenuated interferon alpha-2b fusion constructs may be used to inhibit proliferation in cancerous cells that express both CD38 and the receptor for IFN-alpha2b, as well as to induce apoptosis in such cells. Inhibition of proliferation and induction of apoptosis in cancerous cells may serve as the basis for the treatment of the underlying cancer.

The invention discloses a cancer gene mutation and gene spreading detection kit. The detection kit comprises a detection kit body, the detection kit body is provided with a test tube pedestal, a genereaction tank is arranged below the test tube pedestal and used as a temporary reaction tank for extracted cancer cells, a sponge mat support is installed above the test tube pedestal, a sponge mat isinstalled in the sponge mat support, a reagent tube is installed in a reagent tube tank of the sponge mat, high-molecular sponge is adopted by the reagent tube tank and has stable support and anti-collision effects, and one side of the reagent tube is provided with a reagent label to label a detection reagent name in each reagent tube to enable a reagent to be explicitly classified. A sealed genedetection kit is adopted to prevent bacteria in the air from interfering with a gene detection result, and meanwhile, the detection reagent is prevented from overflowing as well.

The invention relates to a minicircle DNA carrier expressing IgG antibody, a preparation method and application thereof, in particular to a minicircle DNA carrier for in vivo expression of IgG antibody with complete functions. The IgG antibody neutralizes human immunodeficiency virus (HIV), other pathogene antigen or cancer cell antigen. The minicircle DNA carrier and the IgG antibody can be usedfor preventing and treating diseases, such as acquired immune deficiency syndrome (or HIV infection), other pathogen infection or cancers.

The invention discloses a cancer cell detection device based on a sliding window. The cancer cell detection device based on the sliding window comprises a computer, and the computer is programmed to execute the following steps of collecting image data through a cancer cell sampling needle buried in a patient body; after the collection is finished, carrying out fluorescence dyeing treatment and imaging on the data collected on the needle to obtain an image for detection; processing and judging image data; and marking the position of the cancer cell in the original image by using a square frameaccording to the position information obtained in the judgment process, and displaying a final detection result. According to the invention, the sliding window is adopted to intercept the image, so that the calculation complexity caused by inputting the whole image into a network is avoided, the parameter adjustment problem required by preprocessing and feature extraction of the image is also avoided, the operation is accelerated, the accuracy is improved, and the method has universality.

The invention discloses an application of an alkyl indolone derivative and a drug resisting thyroid cancer. The alkyl indolone derivative has a remarkable cancer cell inhibition effect when applied topreparation of anti-cancer drugs. The drug resisting thyroid cancer contains the alkyl indolone derivative with concentration being 0.025 mu M or higher and has remarkable thyroid cancer resistant effect.

The invention discloses a traditional Chinese medicine composition for treating all cancers, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 10-20 parts of oldenlandia diffusa, 25-30 parts of solidago virgaurea, 15-25 parts of scutellaria barbata, 5-15 parts of lobelia chinensis and 15-20 parts of spica prunellae. The traditional Chinese medicine composition is prepared from the pure Chinese herbal medicines as raw materials, contains no hormones or any additives, and solves the problem of large toxic and side effects of a conventional traditional Chinese medicine composition. The traditional Chinese medicine composition for treating all cancers has the advantages of safe and effective treatment of various cancers and little toxic and side effects, can regulate the symptoms of systemic chronic diseases of cancer. Firstly, the traditional Chinese medicine composition guarantees that the spleen and stomach of middle jiao arein harmony with qi to generate wealth, can let cancer cells enter the cancer state, withdraws occupied territories (viscera) and returns to a local area, restores the original state of the cancer cells, makes use of strengthening body resistance and eliminating evil, letting evil return to the right, communicating Yin and Yang, removing relationship of generation-inhibition in five elements, harmonizing Yin and Yang, and harmonizing Qi and blood, and solves the cancer state, so as to achieve the purposes of regulating and conditioning the environment of the cancer cells and curing cancer.

The data provided herewith show that, biomarkers found within the cells of a tumor or cancer may also be present at adequate levels within the patient's urine to enable detection. Method of diagnosis, patient selection and treatment are provided, along with kits and devices.

This invention relates to a complex for cancer cell chemotherapy and, more particularly, to a HER2 aptamer-anticancer drug complex for chemotherapy of cancer cells, which includes a nucleic acid aptamer specifically binding to HER2 and an anticancer drug linked with the nucleic acid aptamer, so that HER2-positive breast cancer cells are selectively targeted and killed.

The invention relates to a nanoprobe capable of analysis and detection of activity of cancer marker MicroRNAs and MicroRNAs in cancer cells and a preparation method therefor. The nanoprobe is a novel four-helical DNA molecule probe. The nanoprobe contains three functional parts: a target object identification area loaded with special design, a trigger area triggering a cyclic amplification reaction and a template area for a polymerization-shearing amplification reaction. The nanoprobe is introduced into a surface enhanced Raman scattering (SERS) analysis detection technology, a four-helical molecule probe-SERS detection method is developed, and high-sensitivity detection of microRNAs can be achieved. Combined with the aptamer identification technology, the four-helical molecule probe-SERS detection method has good specificity, the sample treatment process is simplified, the selectivity is good, the accuracy is high, and the method is an ideal method for detecting the tumor cell marker microRNAs and microRNAs in cancer cells. The detection method and kit can distinguish cancer cells and normal cells, and has advantages of simple operation, and high-sensitivity and high-specificity detection of the cancer marker microRNAs, and thus the kit has good application prospects.

The invention belongs to the technical field of anti-cancer drug information processing, and discloses a personalized cancer medication database system and an establishment method. The method comprises steps of establishing a cancer type and a cancer staging database; recording data of various cancer drugs, and recording input graphs or curves of data intervening in cancer growth along with estrogen concentration change into the database; marking different types of data with significant differences; and selecting an input mode I and an input mode II to analyze a data result of cancer drug intervention cancer growth. The system comprises a customization module for recording data of growth intervention by various cancer drugs according to tumor tissues or cancer cell samples of a patient. Byutilizing the influence of the estrogen level on the effectiveness of the anti-cancer drug, the anti-cancer drug with the maximum curative effect under the estrogen level of a patient is helped to beselected, the maximum curative effect of the anti-cancer drug on various tumors including breast cancer is assisted, and accurate treatment of the anti-cancer drug on the various tumors including breast cancer is achieved.

The invention provides a method for determining TAT cells. Compared with non-cancer related T cells, the TAT cell is expected to have higher specific binding capacity with cancer cells and higher capacity of killing the cancer cells. Meanwhile, the TAT-TCR sequence contained in the TAT cell can also be used or combined with transcriptome information for cancer screening, and the method is high in sensitivity and specificity. Only the peripheral blood sample of a subject needs to be collected, the invasive property is small, more information can be utilized, an existing tumor liquid biopsy method can be well supplemented, early-stage asymptomatic tumors are potentially detected, cancer diagnosis and treatment are integrated, and important application value is achieved.

The present invention is related to an agent for differentiating hematoietic stem cell into natural killer cell comprising VDUP1 protein or gene encoding the same, and a method of differentiating hematopoietic stem cell into natural killer cell using thereof. The present invention reveals for the first time that the VDUP1 gene is a critical factor for the regulation of differentiation of natural killer cell by generating a mouse deficient in VDUP1 gene, which confirms that VDUP1 gene is required for NK maturation. Thus, by the regulation of VDUP1 gene, the modulation of NK cells that have ability to kill cancer cells is possible and can be utilized for cell therapeutics.

The present description provides a cancer assay panel for the targeted detection of cancer-specific methylation patterns. Further provided herein includes methods of designing, making, and using the cancer assay panel to detect cancer and particular types of cancer.