4 results about "Cancer Cellularity" patented technology

The invention is applicable to the technical field of cell type annotation, and provides an interpretable single cell annotation method, which comprises the following steps: based on a source cancer data set, performing cell type label prediction on a target cancer data set through a first module of an annotation model to obtain a prediction label of each cell in the target cancer data set, the annotation model is constructed by adopting an information bottleneck-based graph interpretation technology, and based on the health data set and the prediction label, interpretability analysis is performed on the to-be-interpreted cancer data set through a second module of the annotation model to obtain an interpretation result, the to-be-explained cancer data set is composed of the cancer cell gene expression matrix and the prediction label of the target cancer data set, so that the explanatory mechanism is integrated into the model architecture, the prediction accuracy is ensured, the explanatory stability and reliability are improved, the inconsistency caused by dependence on a post explanatory method is avoided, and the prediction accuracy is improved. And the prediction logic of the model can be understood.

The application discloses a method for predicting different metabolic states of cells in cancer and metabolic characteristic subtypes of the cells based on a metabolite-protein interaction network, and the method comprises the following steps: (1) identifying the classification and metabolic state of the cells: preprocessing data, filtering low-quality cells; performing twice principal component analysis according to key genes; performing twice dimension reduction clustering on the cells respectively; (2) predicting metabolic characteristic subtypes of a queue according to the proportion of different metabolic states of cells in the sample. The method can identify the metabolic state of the cells and metabolic subtypes, and provides a new perspective for cancer research.

The present invention belongs to the field of fluorescent probes, and specifically relates to a specific targeting lipid raft fluorescent probe and its preparation method and application. In view of the characteristics of visualization, non-invasiveness, high sensitivity, etc., fluorescence technology has become an indispensable means of modern biological and medical research, and has played an increasingly important role in the early diagnosis of tumors and surgical navigation. Lipid rafts are microdomains rich in cholesterol and sphingomyelin on the plasma membrane, which play a key role in cancer cell adhesion and migration, and are overexpressed on the membranes of many cancer cells. Therefore, the present invention has developed a probe that can specifically target lipid rafts. The probe can use the characteristic that cancer cells overexpress lipid rafts compared to normal cells to achieve the distinction between the two, and further serve as a fluorescent surgical navigation reagent to guide the removal of tumors in SKOV3 tumor-bearing mice. In addition, the reagent also has the characteristics of short action time and no need for washing.

The invention provides a use of deuterium-labeled fumaric acid or a salt thereof for detecting cancer cell death. The invention provides an application of deuterium-labeled fumaric acid or a salt thereof in preparation of a medicine or a kit. The medicine or the kit is used for detecting death of cancer cells in a subject, detecting a tumor position in the subject or screening an anti-cancer medicine.