Multifunctional nanometer bamboo charcoal environment-friendly printing ink, a preparing method thereof and applications of the printing ink are disclosed. The printing ink includes nanometer bamboo charcoal powder, carbon black, puffed corn starch, tourmaline powder, talcum powder, ground calcium carbonate, montmorillonite powder, nanometer calcium carbonate, nanometer titanium dioxide, hydroxypropyl methyl cellulose and purified water. The printing ink has a high negative ion generating amount and a high far-infrared emissivity, can rapidly improve quality of air around a reader, and can absorb harmful gas, such as volatile organic compounds, and odor generated by other printing ink materials and other products. The wavelength of emitted infrared ray can change microcirculation of readers, protect eyesight, boost immunity, enhance memory and make a reader happy. In addition, the printing ink has automatically sterilizing, mildew-proof, moisture-proof and pest-proof functions and the like, and is particularly suitable for child books, food package printing and archive record printing.