This invention relates to an
abalone active
peptide with the
amino acid sequence DYPRPW. The preparation method is as follows:
Abalone viscera are enzymatically hydrolyzed using
alkaline protease to obtain an enzymatic
hydrolysate; the
enzyme is then inactivated after
hydrolysis. The
hydrolysate is centrifuged, coarsely filtered, and ultrafiltered to collect multiple polypeptides with a molecular weight less than 1 kDa, which are then freeze-dried into
powder. The freeze-dried polypeptide powders are sequenced to obtain multiple polypeptide sequences;
repetitive sequences are deleted, and multiple non-repetitive polypeptides are screened. Using the
protein HMGR as a
receptor, the obtained polypeptides are virtually screened using docking
software, and the polypeptides with the highest docking scores are selected for
solid-
phase synthesis to obtain the
abalone active
peptide. This invention's
abalone active
peptide can effectively inhibit the accumulation of
total cholesterol and total triglycerides in cells, exhibits a high inhibition rate of
cholesterol micelle solubility, and has lipid-lowering effects. It can be used to prepare
adjuvant lipid-lowering drugs, and compared with traditional lipid-lowering drugs, it has the advantages of high specificity and fewer toxic side effects.