Cornel extract and use thereof

A technology of Cornus officinalis and its extracts, which is applied in the field of preparing medicines for treating pain, its active ingredients, autoimmune diseases or inflammations, Cornus officinalis extracts, and can solve the problems of undisclosed components and proportions of Cornus officinalis extracts

Inactive Publication Date: 2008-02-06
HUTCHISON MEDIPHARMA ENTERPRISES LTD
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the patent does not disclose the composition and proportion of the extract of Cornus officinalis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cornel extract and use thereof
  • Cornel extract and use thereof
  • Cornel extract and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Preparation of Cornus officinalis Extract and Determination of Polyphenol Content Therein by Skin Powder Method

[0054] Cornus officinalis 500g, add water 5000mL, decoct 3 times, the degree of decoction is slightly boiled, the decoction time is 2 hours each time, the decoction liquid is filtered, combined, and set aside for later use; The ratio of 1:2 is packed into the column, and then the above-mentioned water decoction is passed through the macroporous adsorption resin column at a flow rate of 20 ml / min. After passing through completely, the resin column is eluted with water, 10% ethanol and 95% ethanol . The eluate was collected and vacuum-dried to obtain 48 g of Cornus officinalis extract.

[0055] Take 2.0076g (W) of the above-mentioned Cornus officinalis extract, dissolve it in 500mL of water, take 25mL of the solution, concentrate it to dryness, and then dry it at 105°C for 3 hours to obtain a solid product, the weight of which is T 1 = 0.0966g.

...

Embodiment 2

[0059] Example 2 Separation and Identification of Three Active Components of Cornus officinalis

[0060] Isolation and identification of loganin:

[0061] 100g of Cornus officinalis can be soaked three times in 500mL of 95% ethanol, and the extracts can be combined to obtain 10g of concentrate. The concentrate was subjected to vacuum column chromatography and eluted with petroleum ether, chloroform and methanol in sequence. After concentration of methanol eluent, silica gel column chromatography (eluent CH 2 CL 2 / CH 3 OH=6:4), to get loganin 875mg, its Rf value=0.57 (developing agent: 0.1M KH 2 PO 4 : 0.1MH 3 PO 4 :CH 3 CN=7:7:4). Its spectral data are:

[0062] [α] D 25 =-81°(c 0.1, H 2 O), UV(λ max , nm) 238 (4.0); IR: (cm -1 , KBr): 3350, 3300, 1715, 1650, 1440, 1300, 1080;

[0063] 1 H NMR (D 2 O, 300MHz) δppm: 7.42 (s, H-3), 5.39 (d, J=3.6Hz, H-1), 4.14 (m, H-7), 3.73 (s, OCH3), 3.05 (m, H -5), 2.16(m, H-6a), 2.10(m, H-9), 1.88(m, H-8), 1.73(m, H-6b), ...

Embodiment 3

[0075] Example 3 HPLC chromatogram and quantitative analysis of Cornus officinalis extract

[0076]Quantitative analysis of Cornus officinalis extract (Example 1) was carried out by HPLC system. Instrument: Agilent 1100, the set wavelength is 240nm, the analytical column is Zorbax SC184.6*150mm, and the flow rate is 1ml / min. The mobile phase is CH 3 CN and 0.1% H 3 PO 4 solution. The gradient status is: CH 3 CN increments from 0% to 25% in 50 minutes. During the next 5 minutes, CH 3 CN is incremented to 100%. Obtain the HPLC collection of illustrative plates shown in accompanying drawing 1.

[0077] The reference substances of loganin, morroniside, and corniside were all isolated and purified in this laboratory. Through HPLC analysis, the purity is above 96%.

[0078] The Cornus officinalis extract of the present invention (Example 1) contains 9.7% loganin (retention time 20.4 minutes), morroniside 17.5% (retention time 26.5 minutes), and corniside 2.6% (retention ti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a cornel extract whose active compositions are morroniside and cornel, and their use in the preparation of medicinal preparation for treating or preventing aches or inflammation.

Description

technical field [0001] The invention relates to a plant extract, in particular to a cornus officinalis extract, its active ingredients and their use in the preparation of medicines for treating pain, autoimmune diseases or inflammation. Background technique [0002] Pain is a common condition in everyday life. According to statistics, about 55% of adults aged 35-50 will suffer from various diseases, such as headache, shoulder and back pain, waist and leg joint pain, soft tissue pain, sciatica, etc. During the development of the disease, it will be accompanied by different degrees of pain in stages, and its intractability and multiples seriously affect the work and life of patients. [0003] Studies have confirmed that the normal immune regulation function of healthy individuals can coordinate autoimmune tolerance and autoimmunity at a complementary and reasonable level. When some reason makes the autoimmune response too strong, it will lead to the damage or dysfunction of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/40A61K31/7048A61P29/00A61P37/02C07H17/04C07H1/08A61P37/00
CPCC07H1/08C07H17/04A61K31/7048A61P29/00A61P37/00A61P37/02A61K2300/00
Inventor 杜莹潘柯严孝强张维汉
Owner HUTCHISON MEDIPHARMA ENTERPRISES LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap