Gamma-polyglutamic acid generating bacterium and process of preparing gamma-polyglutamic acid therewith
A technique for producing polyglutamic acid and bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of expensive materials and achieve the effect of reducing the cost of raw materials
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Embodiment 1
[0029] Get the slant seed preserved in the refrigerator for 1 day [composition of the preserved slant medium (g / L): peptone 10, NaCl 10, yeast extract 5, agar 20, pH7.0. ], use an inoculation needle to inoculate about 1cm on the above slope 2 The bacterial classification of area is scraped off, transfers on the fermentation medium of new configuration, and the composition (g / L) of fermentation medium is as follows: 80g fructose, 18g NH 4 Cl, 1.5g K 2 HPO 4 , 0.05gMgSO 4 ·7H 2 O, 0.01g FeCl 3 ·6H 2 O, 0.15g CaCl 2 2H 2 O, 0.05g MnSO 4 ·H 2 O, and adjust the pH value of the medium (before sterilization) to 6.5 with NaOH and HCl, and sterilize at 120°C for 20 minutes. The fermentation medium was packed in a 500ml Erlenmeyer flask with a capacity of 20ml. It was cultured on a reciprocating shaker with a stroke of 76mm and a speed of 130r / m. After culturing at a constant temperature of 37°C for 96 hours, after the shake flask was fermented, the fermentation broth was used...
Embodiment 2
[0031] Get the slant seed preserved in the refrigerator for 2 days [composition (g / L) of the preserved slant medium: peptone 8, NaCl 11, yeast extract 6, agar 20, pH7.0 in 4°C refrigerator. ], use an inoculation needle to inoculate about 1cm on the above slope 2 The bacterial classification of area is scraped off, is transferred on the fermented medium of new configuration, and the composition (g / L) of fermented medium is as follows: 90g sucrose, 20g (NH 4 ) 2 SO 4 , 1.0g K 2 HPO 4 , 0.06g MgSO 4 ·7H 2 O, 0.01g FeCl 3 ·6H 2 O, 0.15g CaCl 2 2H 2 O, 0.05g MnSO 4 ·H 2 O, and adjust the pH value of the medium (before sterilization) to 6.5 with NaOH and HCl, and sterilize at 120°C for 20 minutes. The fermentation medium was packed in a 500ml Erlenmeyer flask with a capacity of 20ml, and cultured on a reciprocating shaker with a stroke of 76mm and a speed of 120r / m. After 90 hours of constant temperature cultivation at 37°C, after the shake flask was fermented, the ferme...
Embodiment 3
[0033] Get the slant species preserved in the refrigerator for 3 days at 4°C [composition of the slant culture medium (g / L): peptone 12, NaCl 9, yeast extract 4, agar 20, pH7.0. ], use an inoculation needle to inoculate about 1cm on the above slope 2 The bacterial classification of area is scraped off, transfers on the fermentation medium of new configuration, and the composition (g / L) of fermentation medium is as follows: 60g glucose, 20g NH 4 NO 3 , 1.0g K 2 HPO 4 , 0.01gMgSO 4 ·7H 2 O, 0.05g FeCl 3 ·6H 2 O, 0.2g CaCl 2 2H 2 O, 0.104g MnSO 4 ·H 2 O, and adjust the pH value of the medium (before sterilization) to 7.0 with NaOH and HCl, and sterilize at 120°C for 20 minutes. The fermentation medium was packed in a 500ml Erlenmeyer flask with a capacity of 20ml, and cultured on a reciprocating shaker with a stroke of 65mm and a speed of 90r / m. After culturing at a constant temperature of 37°C for 60 hours, after the shake flask was fermented, the fermentation broth w...
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