Continuous free flow electrophoresis device composed of a plurality of specific sub-chambers separated by films

Abstract

The invention discloses continuous free-flow electrophoretic equipment separated by film, which comprises the following parts: main electrophoretic separating chamber, positive and negative electrode chambers and complex protein chamber, wherein the complex protein chamber interconnects inlet and outlet pipes of closed buffer circulating pipe to control transmembrane flow; the buffer updating mechanism contains double-dynamic pump, liquid-container and fresh buffer container with one end extending into inlet and outlet pipes to supplement and drain liquid; the other end of inlet and outlet pipes interconnect liquid feed valve of liquid-supplementing chamber of double-dynamic pump and draining valve of liquid draining chamber; the fresh buffer container and liquid-container interconnect liquid feed valve and liquid exhaust chamber of double-dynamic pump liquid-supplementing chamber; the device can separate and purify protein according to isoelectric point and molecular weight.

Description

Technical field: [0001] The invention relates to a continuous free-flow electrophoresis device for separating protein bioactive substances. Background technique: [0002] Continuous free-flow electrophoresis is a preparative-scale electrophoresis method that can continuously inject samples and continuously collect products. Currently, continuous free-flow electrophoresis for separating proteins mainly includes free-flow zone electrophoresis and free-flow isoelectric focusing. [0003] In free-flow zone electrophoresis, the protein-containing liquid is continuously added to one end of the separation chamber from the direction perpendicular to the electric field, and the proteins with different mobility in the sample obtain different migration speeds parallel to the direction of the electric field and The same moving speed perpendicular to the direction of the electric field is obtained under the continuous input of the liquid flow, so the proteins with different mobility will...

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Problems solved by technology

However, this invention only considers the transmembrane fluid flow caused by the pressure difference, ignoring the transmembrane electroosmotic flow, and it is not perfect to use the height of the liquid level in the balance tank to suppress the transmembrane fluid flow between A and B chambers

In addition, ordinary peristaltic pumps cannot accurately control the liquid flow rate. The liquid flow rate is not only controlled by the speed of the pump head and the inner diameter of the silicone tube, but also by the degree of compression of the silicone tube on the pump head and the deformation of the silicone tube after repeated use. Therefore, it is difficult to control the output speed of the liquid with a peristaltic pump to make it equal to the input speed. Even if it is adjusted for a while, the effect is still unstable

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